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Wang Huan,Hu Zhangxi,Chai Zhaoyang,Deng Yunyan,Zhan Zifeng,Tang Ying Zhong. 2020. Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community. Acta Oceanologica Sinica, 39(4):110-119
Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community
东海原甲藻(Prorocentrum donghaiense)藻华降低了甲藻群落的物种多样性
Received:November 24, 2018  
DOI:10.1007/s13131-020-1585-1
Key words:Prorocentrum donghaiense  dinoflagellate community  diversity  pyrosequencing  East China Sea
中文关键词:  东海原甲藻  甲藻群落  多样性  高通量测序  中国东海
基金项目:The National Natural Science Foundation of China under contract Nos 61533011 and 41776125; the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences under contract No. U1606404; the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology under contract No. 2016ASKJ02; the National Key R&D Program of China under contract No. 2017YFC1404300; the Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology under contract No. LMEES-CTSP-2018-1.
Author NameAffiliationE-mail
Wang Huan CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
University of Chinese Academy of Sciences, Beijing 100049, China 
 
Hu Zhangxi CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China 
 
Chai Zhaoyang CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China 
 
Deng Yunyan CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China 
 
Zhan Zifeng Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China  
Tang Ying Zhong CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China 
yingzhong.tang@qdio.ac.cn 
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Abstract:
      Most of reported harmful algal blooms (HABs) of microalgae (75%) have been caused by dinoflagellates. Studies on the negative effects of HABs have generally focused on animals, valuable organisms in particular, and environmental factors such as dissolved oxygen and nutrients, but relatively fewer on community level, particularly that using metagenomic approach. In this study, we reported an investigation on the effects of a HAB caused by the dinoflagellate Prorocentrum donghaiense on the species diversity and community structure of the dinoflagellate sub-community via a pyrosequencing approach for the samples taken before, during, and after the bloom season of P. donghaiense in the East China Sea. We sequenced partial 28S rRNA gene of dinoflagellates for the field samples and evaluated the species richness and diversity indices of the dinoflagellate community, as a sub-community of the total phytoplankton. We obtained 800 185 valid sequences (categorized into 560 operational taxonomic units, OTUs) of dinoflagellates from 50 samples and found that the biodiversity of dinoflagellate community was significantly reduced during the blooming period in comparison to that in pre- and after-blooming periods, as reflected in the four diversity indices: the species richness expressed as the number of OTUs, Chao1 index, Shannon index (evenness), and Gini-Simpson index. These four indices were all found to be negatively correlated to the cell density of the bloom species P. donghaiense. Correlation analyses also revealed that the P. donghaiense cell abundance was correlated negatively with ${\rm{NO}}_3^- $-N, and ${\rm{NO}}_2^- $-N, but positively with total nitrogen (TN) and total phosphorus (TP). Principal coordinates analysis (PCoA) showed that the community structure of dinoflagellates was markedly different among the different sampling periods, while the redundancy analysis (RDA) revealed P. donghaiense abundance, salinity, ${\rm{NO}}_3^- $-N, and ${\rm{SiO}}_3^{2-} $ were the most four significant factors shaping the dinoflagellate community structure. Our results together demonstrated that HABs caused by the dinoflagellate P. donghaiense could strongly impact the aquatic ecosystem on the sub-community level which the blooming species belongs to.
中文摘要:
      过去几十年,全球各地沿岸水体越来越多地受到由微藻引起的有害藻华(Harmful algal blooms, HABs)的影响,其中大多数(75%)的有害藻华是由甲藻引起的。关于有害藻华对生态系统负面影响的研究通常集中在动物特别是有重要经济价值的生物,以及其它环境因素如溶解氧和营养盐等,但却很少关注有害藻华对群落水平的影响。在本研究中,我们针对中国东海重要藻华原因种东海原甲藻,采集了该藻藻华爆发前、中、后期的样品,引入高通量测序和生态学统计方法,对各样本进行28S rRNA基因(D1-D2区)测序,计算甲藻群落的物种丰富度和多样性指数,以研究东海原甲藻藻华对甲藻群落的物种多样性和结构的影响。我们从50个样本中获得了800,185个有效序列(归类为560个分类单元(OUT, Operational taxonomic unit))。分析发现藻华爆发时甲藻群落的生物多样性显著低于爆发前和爆发后。多样性指数(包括以OTU数量表示的物种丰富度和Chao1指数,Shannon指数(均匀度)及Gini-Simpson指数)与形成藻华的优势种东海原甲藻的细胞丰度呈负相关。通过相关分析还发现东海原甲藻的细胞丰度与NO3--N和NO2--N呈负相关,与总氮(TN)和总磷(TP)呈正相关。主成分坐标分析(PCoA)显示,不同采样时期甲藻的群落结构存在显著差异,而冗余分析(RDA)显示东海原甲藻的丰度、盐度、NO3--N和SiO32-是影响群落结构最重要的4个因素。我们的研究结果表明东海原甲藻藻华对生物多样性的影响可以反映在甲藻亚群落水平上。
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