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Chen Quanrui,Fan Jingfeng,Su Jie,Ming Hongxia,Sun Zhihao,Li Mengfei,Zhao Xiaohui,Wang Yantao,Zhang Yingxue,Zhang Huizhen,Jin Yuan,Ma Xiaowan,Wang Bin. 2020. Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea. Acta Oceanologica Sinica, 39(2):69-78
Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea
渤海沉积物中细菌群落结构和基因丰度的空间分布特征
Received:September 20, 2018  
DOI:10.1007/s13131-020-1554-8
Key words:Bohai Sea sediments  bacterial community structure  high-throughput sequencing  16S rRNA gene
中文关键词:  渤海沉积物  细菌群落结构  高通量测序  16S rRNA基因
基金项目:The National Key Basic Research Special Foundation of China under contract No. 2017YFC1404500; the National Natural Science Foundation of China under contract No. 41676115.
Author NameAffiliationE-mail
Chen Quanrui National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Fan Jingfeng National Marine Environmental Monitoring Center, Dalian 116023, China
Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China 
jffan@nmemc.org.cn 
Su Jie National Marine Environmental Monitoring Center, Dalian 116023, China  
Ming Hongxia National Marine Environmental Monitoring Center, Dalian 116023, China  
Sun Zhihao National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Li Mengfei National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Zhao Xiaohui National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Wang Yantao National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Zhang Yingxue National Marine Environmental Monitoring Center, Dalian 116023, China
College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China 
 
Zhang Huizhen National Marine Environmental Monitoring Center, Dalian 116023, China  
Jin Yuan Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China  
Ma Xiaowan Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China  
Wang Bin College of Aquaculture and Life, Dalian Ocean University, Dalian 116023, China  
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Abstract:
      This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea. Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea. The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury. The main phyla of bacteria recorded included Proteobacteria (52.92%), Bacteroidetes (11.76%), Planctomycetes (7.39%), Acidobacteria (6.53%) and Chloroflexi (4.97%). The genus with the highest relative abundance was Desulfobulbus (4.99%), which was the dominant genus at most sampling stations, followed by Lutimonas and Halioglobus. The main factors influencing bacterial community structure were total organic carbon, followed by depth and total phosphorus. The content of lead, cadmium, chromium, copper and zinc had a consistent effect on community structure. Arsenic showed a negative correlation with bacterial community structure in most samples, while the impact of mercury on community structure was not significant. The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes. The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.
中文摘要:
      本研究调查了渤海沉积物中细菌群落结构对不同环境的响应差异。利用Illumina高通量测序技术和qPCR技术在渤海13个采样站的表层沉积物中检测了细菌16S rRNA基因。结果表明,在13个站位中,大多数沉积物被重金属汞所污染。沉积物中的主要菌群包括变形菌门Proteobacteria(52.92%),拟杆菌门Bacteroidetes(11.76%),浮霉菌门Planctomycetes(7.39%),酸杆菌门Acidobacteria(6.53%)和绿弯菌门Chloroflexi(4.97%)。相对丰度最高的菌属是脱硫叶菌属(4.99%),其在多数采样站中是优势菌,其次是LutimonasHalioglobus。影响细菌群落结构的主要因素是总有机碳,其次是深度和总磷。重金属中铅,镉,铬,铜和锌的含量对群落结构具有一致的影响。在大多数样品中,砷与细菌群落结构呈负相关,而汞对群落结构的影响较弱。渤海沉积物样本中的细菌群落具有丰富的多样性,并且显示出从高纬度到低纬度的多样性增加。数据表明,渤海具有丰富的细菌资源,并且可能代谢多种污染物。
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