| Yan Wenjie,Liu Jiao,Seng Samphal,Zhou Bin,Ding Kuke. 2019. Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina. Acta Oceanologica Sinica, 38(10):111－115 |
| Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina |
| 大叶藻微粒体GST基因对温度胁迫响应的分子机制 |
| Received:May 20, 2018 |
| DOI：10.1007/s13131-019-1429-z |
| Key words:Zostera marina|antioxidant enzyme|glutathione S-transferase (GST)|temperature|enzyme activity |
| 中文关键词: 鳗草(Zostera marina)|抗氧化酶|谷胱甘肽S-转移酶(GST)|温度|酶活性 |
| 基金项目:The Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology, State Oceanic Administration under contract No. 201704; the Shandong Provincial Natural Science Foundation of China under contract No. ZR2018MD020. |
| Author Name | Affiliation | E-mail | | Yan Wenjie | Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | | | Liu Jiao | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | Seng Samphal | Faculty of Fisheries & Aquaculture, Royal University of Agriculture, Phnom Penh 12101, Cambodia | | | Zhou Bin | Department of Marine Ecology, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China | | | Ding Kuke | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China | shouding@ccmu.edu.cn |
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| Abstract: |
| The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express ZmGST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn. |
| 中文摘要: |
| 为了分析鳗草（Zostera marina）中微粒体谷胱甘肽S-转移酶在不同温度下的基因表达规律以及响应机制，本文通过在大肠杆菌中表达ZmGST，纯化重组蛋白以及热稳定性分析，以此为进一步阐述Z. marina的种群退化机制并提供理论依据。总之，鳗草中的微粒体GST的热稳定性和其对温度变化的响应决定了其对温度的耐受范围，并进而影响其恢复力。 |
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