| Gao Wei,Gao Xiangxing,Mi Tiezhu,Han Bin,Zhang Yiran,Li Xinzi,Yin Xiaofei,Sun Chengjun,Li Qian,Cui Zhisong,Luan Xiao,Yu Zhigang,Zheng Li. 2019. Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China. Acta Oceanologica Sinica, 38(6):54－64 |
| Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China |
| 胶州湾沉积物中石油降解菌的降解能力及多样性研究 |
| Received:February 02, 2018 |
| DOI：10.1007/s13131-019-1353-2 |
| Key words:oil spill biodegradation microbial consortium dominant bacteria |
| 中文关键词: 溢油 生物降解 微生物菌群 优势菌 |
| 基金项目:The National Natural Science Foundation of China under contract Nos 41406127 and 41476103; the Basic Scientific Fund for National Public Research Institutes of China under contract No. 2015T05; the National Natural Science Foundation of China-Shandong Joint Funded Project under contract No. U1406403; the Marine Science and Technology Project of Huangdao District under contract No. 2014-4-20; the 2012 Taishan Scholar Award and China-ASEAN Maritime Cooperation Fund East Asia Marine Cooperation Platform. |
| Author Name | Affiliation | E-mail | | Gao Wei | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Gao Xiangxing | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
National Deep Sea Center, Qingdao 266237, China | | | Mi Tiezhu | College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China | | | Han Bin | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Zhang Yiran | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Li Xinzi | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Yin Xiaofei | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Sun Chengjun | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Li Qian | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Cui Zhisong | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Luan Xiao | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China | | | Yu Zhigang | Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China | | | Zheng Li | Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China | zhengli@fio.org.cn |
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| Abstract: |
| A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oil-degrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems. |
| 中文摘要: |
| 2013年青岛输油管道爆炸，大量石油污染了附近海岸。课题组采集了污染的沉积物样品，以原油为唯一碳源和能源，富集了四个石油降解菌群。生物多样性和群落分析表明，Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌，都属于变形菌门。从石油降解菌群中分离筛选，获得了9株具有不同16S rRNA基因序列的降解菌，分别属于8个属。重量法测定降解菌的石油降解率，其中5株的石油降解率大于30%。GC-MS分析结果表明，石油降解菌多倾向于降解烷烃，对多环芳烃的降解能力较差，其中5株细菌的烷烃降解率较大，仅1株菌D2对多环芳烃的降解率较大，其降解率在34.9%到77.5%。通过对高通量数据的分析，表明食烷菌属是菌群A和菌群E的主要降解菌群，其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力，为油污染海滩生物修复提供了菌株资源。 |
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