| Li Wanjuan,Zhao Huan,Ba Fuyang,Li Shaojuan,Sun Xiupeng,Yang Dazuo,Zhou Yibing. 2019. mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene. Acta Oceanologica Sinica, 38(6):46－53 |
| mRNA expression of CYP4 in marine polychaete Marphysa sanguinea in response to benzo[a]pyrene |
| 苯并（a）芘诱导下岩虫CYP基因的表达特征 |
| Received:June 06, 2017 |
| DOI：10.1007/s13131-019-1362-1 |
| Key words:Marphysa sanguinea CYP4 benzo[a]pyrene toxicity effect |
| 中文关键词: 岩虫 CYP4 苯并(a)芘 毒性效应 |
| 基金项目:The Dalian Science Fund for Distinguished Young Scholars under contract No. 2016RJ06; the Natural Science Foundation of Liaoning Province under contract No. 201602107; the Superior College Science Technology Research Project of Liaoning Province under contract No. ZL201701; the National Natural Science Foundation of China under contract No. 41306138. |
| Author Name | Affiliation | E-mail | | Li Wanjuan | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | | | Zhao Huan | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | | | Ba Fuyang | Marine and Fisheries Technology Center of Pan Shan Country, Panjin 124000, China | | | Li Shaojuan | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | | | Sun Xiupeng | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | | | Yang Dazuo | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | dzyang@dlou.edu.cn | | Zhou Yibing | Key Laboratory of Marine Bioresource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China | ybzhou@dlou.edu.cn |
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| Abstract: |
| Rapid amplification of cDNA ends (RACE) and real-time polymerase chain reaction (RT-PCR) were carried out to analyze the CYP4 gene expression in polychaete Marphysa sanguinea exposed to benzo[a]pyrene (BaP) in this study. The full length of MsCYP4 cDNA was 2 470 bp, and it encoded 512 amino acids. The deduced amino acid sequence showed 47% identity with CYP4F from frog Xenopus tropicalis and shared high homology with other known CYP4 sequences. To analyse the role of CYP4 in protecting M. sanguinea from BaP exposure, three BaP groups were established:0.5, 5 and 50 μg/L. Polychaetes were sampled after 3, 7 and 12 d. At 0.5 μg/L, the effect of BaP on MsCYP4 gene expression increased with time prolonged. MsCYP4 gene expression curve showed U-shaped trend with time in 5 and 50 μg/L BaP groups. Therefore, MsCYP4 gene may play an important role in maintaining the balance of cellular metabolism and protecting M. sanguinea from BaP toxicity. |
| 中文摘要: |
| 本文利用RACE技术首次获得岩虫CYP4 cDNA序列全长。结果显示岩虫CYP4 cDNA全长2470bp，编码512个氨基酸。该蛋白序列与已知CYP4序列同源性较高，与热带爪蟾Xenopus tropicalis的序列同源性达到47%。利用实时荧光定量PCR技术检测了不同浓度苯并（a）芘（0.5，5 和50μg/L）诱导下岩虫CYP4基因表达变化特征。结果显示苯并（a）芘暴露会诱导岩虫CYP4基因表达。在0.5μg/L浓度组岩虫CYP4基因表达随诱导时间的延长而上调，但在5 和50μg/L浓度组中基因表达与诱导时间呈现先升复降后升的U形变化。这一结果表明岩虫CYP4在外源污染物解毒代谢中具有一定作用。 |
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