| Jin Min,Chen Chen,He Xiongfei,Zeng Runying. 2019. Characterization of an extreme alkaline-stable keratinase from the draft genome of feather-degrading Bacillus sp. JM7 from deep-sea. Acta Oceanologica Sinica, 38(2):87－95 |
| Characterization of an extreme alkaline-stable keratinase from the draft genome of feather-degrading Bacillus sp. JM7 from deep-sea |
| 深海羽毛降解菌Bacillus sp. JM7极端碱耐受角蛋白酶的酶活性质研究 |
| Received:May 04, 2017 |
| DOI：10.1007/s13131-019-1350-5 |
| Key words:Bacillus deep-sea feather-degradation keratinases feather bioconversion alkaline-stable |
| 中文关键词: 枯草芽孢杆菌 深海 羽毛降解 角蛋白酶 羽毛生物转化 碱稳定性 |
| 基金项目:The Scientific Research Foundation of Third Institute of Oceanography, Ministry of Natural Resources under contract No. 2015019; the National Natural Science Foundation of China under contract No. 41606144; the Science Foundation of the Fujian Province, China under contract No. 2016J05098. |
| Author Name | Affiliation | E-mail | | Jin Min | State Key Laboratory Breeding Base of Marine Genetic Resource
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China | | | Chen Chen | State Key Laboratory Breeding Base of Marine Genetic Resource
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China | | | He Xiongfei | State Key Laboratory Breeding Base of Marine Genetic Resource
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China | | | Zeng Runying | State Key Laboratory Breeding Base of Marine Genetic Resource
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510000, China
Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China | zeng@tio.org.cn |
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| Abstract: |
| Bacillus sp. JM7, a strain isolated from the deep-sea of the South China Sea, was found to efficiently degrade 79.4% native chicken feather within 30 h. Scanning electron microscopy analysis showed that JM7 strain could gradually degrade feather by modifying the microstructure of feather keratin. A total of 25 protease genes were predicted from the draft genome of JM7 strain, among which a predicted subtilisin-like serine protease (designated as Ker02562) was further characterized for its keratinolytic activity. The recombinant Ker02562 functioned at a wide range of temperatures from 30℃ to 60℃, with an optimum at 40-50℃. Ker02562 was highly active at various pHs ranging from 5.0 to 13.0, with a maximum activity observed at pH 7.0-9.0. Remarkably, recombinant Ker02562 was stable in extreme alkaline environments (pH 10-13), which was much better than most other reported keratinases. Collectively, these favorable properties could make Bacillus sp. JM7 and Ker02562 attractive to be applied in the detergent formulation and feather bioconversion. |
| 中文摘要: |
| 从南海深海中分离获得一株可以高效降解羽毛的枯草芽孢杆菌Bacillus sp. JM7，该菌株可以在30小时之内有效地降解79.4%的鸡羽毛。扫描电镜观察发现JM7菌株通过改变羽毛角蛋白的细微结构来逐渐降解羽毛。我们对JM7菌株的基因组序列进行了高通量测序，并从基因组草图中预测获得了25个蛋白水解酶基因。我们选择其中一个预测为丝氨酸蛋白酶的基因（命名为Ker02562），进行进一步的角蛋白酶活性质分析。性质分析结果显示，重组Ker02562蛋白能够在广泛的温度（30°-60℃）和pH（5.0-13.0）范围内发挥活性，其最适反应温度为40-50℃，最适反应pH为7.0-9.0。重要的是，重组Ker02562蛋白能够在极端碱性环境下（pH 10-13）保持稳定，表现出比其他已报道角蛋白酶都要优秀的极端碱耐受能力。这些研究为我们进一步开发Bacillus sp. JM7 菌株和Ker02562蛋白在洗洁精合成和羽毛生物转化方面的用途奠定了基础。 |
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