| Wang Xinzhi,Wu Hao,Cheng Ying,Wen Hongmei,Liu Rui,Wang Libao,Shan Chenxiao,Chai Chuan. 2019. Multi-year assessment of paralytic shellfish toxins in hard clam species along the coastline of Jiangsu Province, China. Acta Oceanologica Sinica, 38(2):24－33 |
| Multi-year assessment of paralytic shellfish toxins in hard clam species along the coastline of Jiangsu Province, China |
| 江苏沿海贝类软体中麻痹性贝毒素种类及含量变化的多年观测研究 |
| Received:June 12, 2018 |
| DOI：10.1007/s13131-019-1347-0 |
| Key words:paralytic shellfish toxin HILIC-MS/MS clam seasonal variation Jiangsu Province |
| 中文关键词: 麻痹性贝毒素 HILIC-MS/MS法 蛤蜊 季节变化规律 江苏沿海 |
| 基金项目:The Public Science and Technology Research Funds Projects of Ocean under contract Nos 201305007 and 201405017. |
| Author Name | Affiliation | E-mail | | Wang Xinzhi | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China | wxzatnj@sina.com | | Wu Hao | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China
Marine Drug Research and Development Center of Jiangsu Province, Nanjing 210023, China | | | Cheng Ying | Marine Drug Research and Development Center of Jiangsu Province, Nanjing 210023, China | | | Wen Hongmei | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China | | | Liu Rui | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China | | | Wang Libao | Marine Fisheries Research Institute of Jiangsu, Nantong 226007, China | | | Shan Chenxiao | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China | | | Chai Chuan | School of Pharmacy, Nanjing University of Chinese Medicines, Nanjing 210023, China | |
|
| Hits: 1197 |
| Download times: 859 |
| Abstract: |
| Paralytic shellfish toxins (PSTs) are notorious neurotoxins that threaten public health and food safety worldwide. Although PST monitoring programs have recently been established throughout China, the profiles and variation of PSTs in important commercial clams (e.g., Mactra veneriformis, Ruditapes philippinarum, and Meretrix meretrix) along the Jiangsu Province coastline remain largely unexplored. In this study, a validated hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was used to examine PST profiles and levels in 540 clam samples from natural production areas along Jiangsu Province coastline during 2014-2016. Although the PST levels (≤ 6.38 μg saxitotoxin equivalents (eq)/kg) were consistently below European Union regulatory limits (≤ 800 μg saxitotoxin eq/kg) during this time period, saxitotoxin, decarbamoylsaxitotoxin, and gonyautoxins 1 and 4 were detected, and nearly 40% of the samples were saxitotoxin-positive. The PST levels also varied significantly by seasons, with peak values observed in May during 2014-2016. This is the first systematic report of PSTs in clams from Jiangsu Province, and additional research and protective measures are needed to ensure the safety of clams harvested in this area. |
| 中文摘要: |
| 麻痹性贝毒素（PSTs）是导致海洋水产品安全问题的重要因素，直接威胁到全球消费者的生命健康。虽然中国部分沿海地区已逐步对海产品中麻痹性贝毒素的含量进行监控，但江苏沿海经济型贝类（如四角蛤蜊、花蛤和文蛤等）中麻痹性贝毒素的类型及其含量随季节性变化的规律目前尚缺乏系统性的研究。本实验在建立高灵敏度亲水相互作用高效液相色谱-串联质谱法 （HILIC-MS/MS）测定江苏沿海贝类中麻痹性贝毒素的基础之上，对采自2014-2016年间江苏东部沿海不同产区、采收季节的540个贝类软体样品中麻痹性贝毒素的种类及含量进行测定。结果显示，2014-2016年间，所采集的贝类中总麻痹性贝毒素水平（≤ 6.38 μg 石房蛤毒素 当量/kg）均低于欧盟监管限制标准（≤ 800 μg 石房蛤毒素 当量/kg），检测样品中共检出石房蛤毒素（saxitotoxin）， 脱氨甲酰石房蛤毒素（decarbamoylsaxitotoxin）， 以及膝沟藻毒素1和4（gonyautoxins 1 & 4）共四种毒素，其中近40%的样品检出含有石房蛤毒素。本实验结果还提示，江苏沿海贝类中麻痹性贝毒素的水平随季节的变化而产生规律性变化，春季相对较高，峰值出现在每年的5月。本文首次对江苏沿海贝类软体中麻痹性贝毒素类成分进行了系统的研究，实验结果将为江苏沿海贝类品质监控以及安全性评价具有较好的科学指导意义，并为江苏海岸麻痹性贝毒素的分布情况以贝类采收季节提供相应的参考依据。 |
|
HTML
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
