| ZHANG Xiaodong,YANG Shuang,SUN Jun,WU Chao,WANG Jing,ZHANG Guicheng,DING Changling. 2018. Morphology, ultrastructure and phylogeny of Cyanothece sp. (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean. Acta Oceanologica Sinica, 37(10):4－10 |
| Morphology, ultrastructure and phylogeny of Cyanothece sp. (Cyanobacteriaceae: Cyanophyceae) isolated from the eastern Indian Ocean |
| 东印度洋分离的一株蓝杆藻的形态学,超显微结构以及系统发生分析 |
| Received:September 19, 2017 |
| DOI：10.1007/s13131-018-1297-y |
| Key words:Cyanothece cyanobacteria morphology 16S rRNA gene nifH gene Indian Ocean |
| 中文关键词: 蓝杆藻 蓝藻 形态学 16S rRNA nifH基因 印度洋 |
| 基金项目:The National Natural Science Foundation of China under contract Nos 41876134, 41676112, 41276124, 41406155 and 41506182; the Natural Science Foundation of Tianjin under contract No. 17JCZDJC40000; the Exploration Program of Ocean with Science and Technology of Tianjin under contract No. KJXH2013-22; the Changjiang Scholar Program of Chinese Ministry of Education of China to Jun Sun. |
| Author Name | Affiliation | E-mail | | ZHANG Xiaodong | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | | | YANG Shuang | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | | | SUN Jun | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | phytoplankton@163.com | | WU Chao | Institute of Marine Science and Technology, Shandong University, Jinan 250110, China | | | WANG Jing | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | | | ZHANG Guicheng | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | | | DING Changling | Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China
Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China | |
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| Abstract: |
| One strain of unicellular greenish algae embedded by mucilage was successfully isolated from equatorial area in the Indian Ocean. Microscopic observation, ultrastructure features and genetic identification confirmed that the strain was closely related to Cyanothece sp., which was a cyanobacteria species with great ecological significance. Cells were solitary with oval or bacilliform shapes. Diameters of this strain were relatively small, ranging from 2.5 to 6.5 μm on average. Ultrastructure of cells was simple. Thylakoids were arranged parietal and keritomized content were observed in the thylakoid region. Various electron-transparent granules with low electron-dense region as well as cyanophycin or glycogen granules-like organelle and carbonxysomes were also observed. For pigment composition, the dominant pigments were chlorophyll a, β-Carotene, Zeaxanthin and an unknown pigment, contributing 23.8%, 26.1%, 14.7% and 15.7% to total pigments respectively. The phylogenetic analysis of 16S rRNA gene and nifH gene confirmed that Strain EIO409 was closely related with Cyanothece sp.. |
| 中文摘要: |
| 我们在印度洋赤道区域成功分离一株胶质包裹的绿色单细胞藻。结合显微镜观察，超显微结构分析以及分子生物学鉴定，我们将其鉴定为蓝藻门中具有很重要生态学意义的蓝杆藻。这株藻为椭圆形或棍形的单细胞，直径相对较小，平均为2.5到6.5μm。细胞超显微结构较为简单，类囊体贴壁排列，内部有窝状结构。细胞内有许多电子透明的颗粒以及类似藻青素或淀粉粒的细胞器以及羧酶体。色素组成方面，细胞最主要的色素是叶绿素a，β-胡萝卜素，玉米黄素，以及一种未知色素，分别占总色素比例为23.8%，26.1%，14.7% and 15.7%。经过16S rRNA以及nifH基因序列分析，此株系被鉴定为蓝杆藻。 |
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