| ZHANG Pingping,LI Jiang,YU Leiye,WEI Jingfang,XU Tong,SUN Guojie. 2018. Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells. Acta Oceanologica Sinica, 37(8):46－53 |
| Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp. B-3 on gene expression in RAW264.7 macrophage cells |
| 转录组学分析B-3胞外多糖对巨噬细胞RAW264.7基因表达的影响 |
| Received:December 01, 2017 Revised:April 12, 2018 |
| DOI：10.1007/s13131-018-1227-z |
| Key words:Psychrobacter sp. B-3 B-3 exopolysaccharide RAW264.7 macrophage differentially expressed genes immunological pathway metabolic pathway |
| 中文关键词: Psychrobacter sp.B-3 B-3胞外多糖 RAW264.7巨噬细胞系 差异表达基因 免疫通路 代谢通路 |
| 基金项目:The Important National Science & Technology Specific Projects under contract No. 2011ZX8001-003; the National Natural Science Fundation of China under contract No. 40706053; Chinese Polar Environment Comprehensive Investigation & Assessment Programs under contract No. CHINARE2017-01-05. |
| Author Name | Affiliation | E-mail | | ZHANG Pingping | College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China | | | LI Jiang | Key Laboratory of Marine Bioactive Substances, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | lijiang@fio.org.cn | | YU Leiye | Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | WEI Jingfang | College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China | | | XU Tong | College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China | | | SUN Guojie | College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China | sunguojie1975@126.com |
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| Abstract: |
| B-3 exopolysaccharide is extracted from the Antarctic psychrophilic bacterium Psychrobacter sp. B-3. We have previously shown that it activates macrophages and affects their immunoregulatory activities. To determine what genes are affected during this process, we detected the genes differentially expressed in cells of RAW264.7 macrophages treated with B-3 exopolysaccharide by transcriptomic analysis. B-3 exopolysaccharide treatment caused differential expression of 420 genes, of which 178 were up-regulated and 242 were down-regulated. These genes were shown to be involved in many aspects of cell function, mainly metabolism and immunity. Genes were enriched in multiple immune-related pathways, and the most significantly enriched genes were involved in antigen processing and presentation pathways. The pathway in which differentially expressed genes were the most significantly enriched was the metabolic pathway; specifically, the expression of many metabolic enzyme genes was altered by B-3 exopolysaccharide treatment. Additionally, the genes involved in metabolisms of amino acids, carbohydrates, lipids and nucleotides, varied to certain degrees. B-3 exopolysaccharide, therefore, appears to directly affect the immune function of RAW264.7 macrophages as an immunostimulant, or to indirectly change intracellular metabolism. This is the first study to determine the effect of an Antarctic psychrophilic bacterial exopolysaccharide on RAW264.7 macrophages. Our findings provide an important reference for research into the regulation of macrophage immune function by different polysaccharides. |
| 中文摘要: |
| B-3胞外多糖是从一株南极嗜冷菌Psychrobacter sp.B-3发酵液中提取的胞外多糖，前期研究发现该多糖能够激活巨噬细胞并影响其免疫调控活动。为探明其免疫调控途径，采用转录组学分析对经B-3多糖刺激的RAW264.7巨噬细胞系进行了差异表达基因分析。研究结果表明B-3多糖的刺激引起了420个基因的差异表达，其中上调178个、下调242个。差异表达基因与细胞功能存在诸多关联，但主要集中在代谢和免疫两个通路上：其中在多个免疫相关通路上，富集最显著的是抗原处理和表达途径；另一个明显富集的则是代谢通路，B-3多糖处理引起了大量参与代谢的酶类的表达变化，包括氨基酸，碳水化合物，脂质和核苷酸代谢相关基因均发生了一定程度的改变。因此，B-3胞外多糖作为一种免疫激活剂直接影响了RAW264.7巨噬细胞系的免疫功能，同时也对其胞内的代谢途径产生了影响。本论文首次开展了南极嗜冷细菌胞外多糖对RAW264.7巨噬细胞系的影响，该研究为不同来源的活性多糖对巨噬细胞免疫激活研究提供了科学依据。 |
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