| LI Yuan,ZHANG Liyan,SONG Puqing,ZHANG Ran,WANG Liangming,LIN Longshan. 2018. Fish diversity and molecular taxonomy in the Prydz Bay during the 29th CHINARE. Acta Oceanologica Sinica, 37(8):15－20 |
| Fish diversity and molecular taxonomy in the Prydz Bay during the 29th CHINARE |
| 基于29次南极科考的普利兹湾鱼类多样性和分子种类鉴定 |
| Received:July 19, 2017 Revised:May 18, 2018 |
| DOI：10.1007/s13131-018-1228-y |
| Key words:DNA barcoding Prydz Bay Antarctic fish phylogenetic relationship barcoding gap |
| 中文关键词: DNA条形码 普利兹湾 南极鱼类 系统发育关系 条形码间隙 |
| 基金项目:Chinese Polar Environment Comprehensive Investigation and Assessment Program under contract Nos CHINARE 2012-2015-01-05, CHINARE 2012-2015-04-01 and CHINARE 2017-04-03. |
| Author Name | Affiliation | E-mail | | LI Yuan | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | ZHANG Liyan | Fujian Institute of Oceanography, Xiamen 361013, China | | | SONG Puqing | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | ZHANG Ran | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | WANG Liangming | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | LIN Longshan | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | linlsh@tio.org.cn |
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| Abstract: |
| In 2013, the 29th Chinese National Antarctic Research Expedition (CHINARE) prospected the Prydz Bay on the Antarctic continental shelf, and the Chinese R/V Xuelong icebreaker sampled all of the examined locations. The nature of Antarctic fish diversity in the high-latitude Prydz Bay is virtually unknown, and the accuracy of relevant estimates has not been established. Thus, it is necessary to evaluate this diversity and propose protective measures. In total, ninety-nine specimens were collected from various locations. To overcome uncertainties associated with identifying species based on morphology, DNA barcoding (COI gene) was employed to reconstruct phylogenetic relationships with delimited references from NCBI. Twenty-two species representing six families were unambiguously identified from a neighbor-joining (NJ) tree and barcoding gaps. With the morphological identification, thirteen species were identified correctly, five species were identified correctly at the genus level, and four species were identified at the close sister species level. Notothenioid dominance was not evident in the Prydz Bay, in contrast to other published studies. The low species diversity and catch biomass during this CHINARE were severely constrained by limited fishing methods and localized sites, which led to biased underestimation. Our analyses indicate that DNA barcoding is an effective tool for the identification of fish species in the Prydz Bay. The identification and distribution of Antarctic fish should be an integral component of understanding Antarctic fish biodiversity and biogeography, and large-scale studies are necessary for the further taxonomic identification of Antarctic fish. |
| 中文摘要: |
| 利用2013年29次南极科考对南极半岛普利兹湾的调查采集到的鱼类进行多样性和分类鉴定，所有站位的样品均由雪龙号采集。位于高纬度普利兹湾内的鱼类多样性和相关的评估尚未知晓。因此，有必要对该调查海域的鱼类多样性进行准确的评估。本航次共捕获鱼类样品99尾，为了克服形态鉴定不准确的缺点，我们结合DNA条形码（COI基因片段）技术对种类鉴定，同时下载NCBI基因库中的公开数据作为参考。利用NJ系统发育树和条形码间隙共准确鉴定出22种鱼类，其中与形态鉴定相对应的有13种，5种鱼类准确鉴定到属的水平，4种鉴定到其近缘种。公开发表的资料中显示南极鱼科鱼类在种类组成中属于优势种类，但是在本次调查中并未发现这一现象。受调查方法和站位数量的限制，本次调查鱼类表现出较低的多样性和较低生物量，这一结果可能导致对普利兹湾鱼类多样性认识的片面性。结果证明条形码技术可以有效的用于普利兹湾鱼类的种类鉴定，同时南极鱼类的种类鉴定和分布是了解南极鱼类物种多样性和生物地理学的一部分，因此，有必要对南极鱼类在大尺度上对其准确的种类鉴定。 |
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