| PARK Mirye,PARK So Yun,HWANG Jinik,JUNG Seung Won,LEE Juyun,CHANG Man,LEE Taek-Kyun. 2018. Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra. Acta Oceanologica Sinica, 37(5):107－112 |
| Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra |
| Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra |
| Received:August 29, 2017 Revised:December 11, 2017 |
| DOI：10.1007/s13131-018-1167-7 |
| Key words:nuclease protection assay sandwich hybridization Heterocapsa triquetra red tide monitoring |
| 中文关键词: nuclease protection assay sandwich hybridization Heterocapsa triquetra red tide monitoring |
| 基金项目:The Public Welfare & Safety Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning under contract No. NRF-2013M3A2A1067529. |
| Author Name | Affiliation | E-mail | | PARK Mirye | South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea
Bioresources Culture Collection Division, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea | | | PARK So Yun | South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea | | | HWANG Jinik | South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea | | | JUNG Seung Won | South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea | | | LEE Juyun | Research Strategy Team, Korea Marine Environment Management Corporation, Seoul 05718, Republic of Korea | tklee@kiost.ac.kr | | CHANG Man | Research Strategy Team, Korea Marine Environment Management Corporation, Seoul 05718, Republic of Korea | | | LEE Taek-Kyun | South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea | |
|
| Hits: 761 |
| Download times: 792 |
| Abstract: |
| Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5×104 cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0×104 cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions. |
| 中文摘要: |
| Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5×104 cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0×104 cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions. |
|
HTML
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
