| LI Yuan,ZHANG Yan,GAO Tianxiang,HAN Zhiqiang,LIN Longshan,ZHANG Xiumei. 2017. Morphological characteristics and DNA barcoding of Pampus echinogaster (Basilewsky, 1855). Acta Oceanologica Sinica, 36(12):18－23 |
| Morphological characteristics and DNA barcoding of Pampus echinogaster (Basilewsky, 1855) |
| 镰鲳（Basilewsky，1855）形态特征和DNA条形码研究 |
| Received:March 06, 2017 |
| DOI：10.1007/s13131-017-1124-x |
| Key words:morphological characteristics DNA barcoding Pampus echinogaster genetic differentiation transverse occipital canal |
| 中文关键词: 形态特征 DNA条形码 镰鲳 遗传分化 |
| 基金项目:The National Natural Science Foundation of China under contract No. 41776171; the National Programme on Global Change and Air-Sea Interaction under contract Nos GASI-02-SCS-YSWspr/aut and GASI-02-PAC-YDsum/aut; the Scientific Research Foundation of TIO, SOA under contract No. 2016010; the Bilateral Cooperation of Maritime Affairs under contract No. 2200207. |
| Author Name | Affiliation | E-mail | | LI Yuan | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | ZHANG Yan | Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China | | | GAO Tianxiang | National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316004, China | | | HAN Zhiqiang | National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316004, China | | | LIN Longshan | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | ZHANG Xiumei | National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316004, China | xiumei1227@163.com |
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| Abstract: |
| The morphological similarities of Pampus fishes have led to considerable confusion in species-level identification, and no accurate information on neotype or DNA barcoding of Pampus echinogaster is available. Two hundred and seven specimens of P. echinogaster were collected from the coastal waters of Dandong, Dongying, Qingdao, Nantong, Zhoushan, Wenzhou, Changle, Taiwan, and Wakayama (Japan), from June 2010 to April 2013. The diagnostic characteristics of P. echinogaster are as follows: dorsal fin VIII-XI-43-51, anal fin V-VIII-43-49, pectoral fin 22-27, caudal fin 19-22, pelvic fin absent; first gill rakers sparse, slender (pointed), 3-4+12-16=15-20; vertebrae 39-41; transverse occipital canal on top of head moderately small, wavy ridges not reaching upper origin of pectoral fin; ventral branch of lateral line canal spare, shorter than dorsal branch of lateral line canal. By combining congener sequences of the cytochrome oxidase I (COI) gene from GenBank, two absolute groups were detected among all specimens, which further indicated that two valid species were present based on genetic differences in amino acid sequences and the distance between the groups. The sequences of Group 1 can be regarded as DNA barcoding of P. echinogaster. The correct morphological redescription and DNA barcoding of P. echinogaster are presented here to provide a guarantee for efficient and accurate studies, a theoretical basis for classification, and enable appropriate fishery management and conservation strategies for the genus Pampus in the future. |
| 中文摘要: |
| 鲳属鱼类相似的外部形态特征导致其种类鉴定非常混乱，其中镰鲳模式标本的准确信息和DNA条形码更是无法获得。2010年6月到2013年4月期间，我们在丹东、东营、青岛、南通、舟山、温州、长乐、台湾及和歌山县（日本）收集了207尾镰鲳样品。基于上述样品归纳了镰鲳的主要形态鉴别特征为：背鳍Ⅷ-Ⅺ-43~51；臀鳍Ⅴ-Ⅷ-43~49；胸鳍22~27；尾鳍19~22，腹鳍缺失。第一鳃耙细弱，稀疏，3~4+12~16=15-20。脊椎骨数为39~41。头部后上方侧线管的横枕管丛和背分支丛后缘呈弧形，略短，背部横枕管丛末端未达胸鳍上部基点。腹部横枕管丛稀少，明显短于背部横枕管丛。通过比较NCBI上下载的COI同源序列，两个显著的组群被检测到，研究结果显示在氨基酸序列和组群间遗传距离的基础上，该两大组群实为两个有效种，其中组群1的序列可以作为镰鲳的DNA条形码。本论文提出镰鲳正确的外部形态特征和DNA条形码，可为后续鲳属鱼类的研究提供高效、准确的保障，也为其分类提供理论基础，并能鲳属鱼类的渔业管理和保护策略提供合理的参考。 |
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