| QIAO Ying,WANG Jun,MAO Yong,LIU Min,CHEN Ruanni,SU Yongquan,KE Qiaozhen,HAN Kunhuang,ZHENG Weiqiang. 2017. Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Acta Oceanologica Sinica, 36(10):115－119 |
| Pathogenic bacterium Vibrio harveyi:an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans |
| 致病性哈维氏弧菌:海洋寄生性纤毛虫的内共生细菌 |
| Received:April 29, 2016 |
| DOI：10.1007/s13131-017-1050-y |
| Key words:endosymbiotic bacterium protozoan Vibrio harveyi Cryptocaryon irritans Meta16S sequencing |
| 中文关键词: 内共生细菌 原生动物 哈维氏弧菌 刺激隐核虫 16s宏测序 |
| 基金项目: |
| Author Name | Affiliation | E-mail | | QIAO Ying | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | WANG Jun | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | MAO Yong | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | LIU Min | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | CHEN Ruanni | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | SU Yongquan | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | yqsu@xmu.edu.cn | | KE Qiaozhen | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China | | | HAN Kunhuang | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China | | | ZHENG Weiqiang | State Key Laboratory of Breeding of Larimichthys crocea, Ningde 352103, China | |
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| Abstract: |
| Vibrio harveyi, known as a pathogenic bacterium caused severe secondary bacterial infections of the large yellow croaker Larimichthys crocea, was identified as an endosymbiont in the marine parasitic ciliate protozoan Cryptocaryon irritans. Meta 16S sequencing method was used to identify the bacterial flora in C. irritans, and V. harveyi was isolated via culture-dependent method. Vibrio harveyi was observed in cytoplasm of C. irritans at the stage of tomont both by transmission electron microscopy and by Fluorescence in situ hybridization; no signal, however, was detected in nucleus area. The relationship between V. harveyi and C. irritans and the role of endosymbiotic V. harveyi in C. irritans merit further investigation. |
| 中文摘要: |
| 哈维氏弧菌被观察到与海洋寄生性纤毛虫刺激隐核虫具有内共生现象，其作为一种致病性细菌，可导致感染刺激隐核虫的大黄鱼产生严重的继发性细菌感染。我们通过16s宏测序技术对刺激隐核虫的细菌群落进行鉴定，并通过标准的细菌培养方法分离鉴定出哈维氏弧菌。通过透射电镜和荧光原位杂交方法，在刺激隐核虫的细胞胞质中，均观察到内共生的哈维氏弧菌的存在；而在其细胞核中，则没有相关信号存在。哈维氏弧菌与刺激隐核虫的相关关系以及内共生的哈维氏弧菌对刺激隐核虫的影响作用，仍需要深入进行研究。 |
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