| MA Yuexin,TAO Wei,LIU Changfa,LIU Jiao,YANG Zhiping,LI Jin,LIU Jichen. 2017. Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China. Acta Oceanologica Sinica, 36(6):80－88 |
| Response of microbial biomass and bacterial community composition to fertilization in a salt marsh in China |
| 施肥对盐沼沉积物微生物生物量和细菌群落组成的影响 |
| Received:January 16, 2016 Revised:August 02, 2016 |
| DOI：10.1007/s13131-017-1048-5 |
| Key words:fertilization microbial biomass 16S rRNA gene abundance bacterial community salt marsh |
| 中文关键词: 施肥 微生物生物量 16S rRNA基因丰度 细菌群落 盐沼 |
| 基金项目:The National Natural Science Foundation of China under contract No. 41171389; the Public Science and Technology Research Funds Projects of Ocean under contract No. 201305043; Program for Liaoning Excellent Talents in University under contract No. LR2013035. |
| Author Name | Affiliation | E-mail | | MA Yuexin | Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China | mayuexin@dlou.edu.cn | | TAO Wei | Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China | | | LIU Changfa | Key Laboratory of Marine Environmental Research of Liaoning Higher Education, Dalian 116023, China | | | LIU Jiao | Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China | | | YANG Zhiping | Dalian Huixin Titanium Equipment Development Company Limited, Dalian 116039, China | | | LI Jin | Institute of Ocean and Fisheries of Panjin, Panjin 124001, China | | | LIU Jichen | Key Laboratory of Mariculture and Stock Enhancement in North China's Sea of Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China | |
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| Abstract: |
| The effects of nitrogen (N) addition on microbial biomass, bacterial abundance, and community composition in sediment colonized by Suaeda heteroptera were examined by chloroform fumigation extraction method, real-time quantitative polymerase chain reaction, and denaturing gradient gel electrophoresis (DGGE) in a salt marsh located in Shuangtai Estuary, China. The sediment samples were collected from plots treated with different amounts of a single N fertilizer (urea supplied at 0.1, 0.2, 0.4 and 0.8 g/kg (nitrogen content in sediment) and different forms of N fertilizers (urea, (NH4)2SO4, and NH4NO3, each supplied at 0.2 g/kg (calculated by nitrogen). The fertilizers were applied 1-4 times during the plant-growing season in May, July, August, and September of 2013. Untreated plots were included as a control. The results showed that both the amount and form of N positively influenced microbial biomass carbon, microbial biomass nitrogen, and bacterial abundance. The DGGE profiles revealed that the bacterial community composition was also affected by the amount and form of N. Thus, our findings indicate that short-term N amendment increases microbial biomass and bacterial abundance, and alters the structure of bacterial community. |
| 中文摘要: |
| 通过氯仿熏蒸提取法、实时荧光定量PCR和变性梯度凝胶电泳（DGGE）研究了施氮（N）对双台河口翅碱蓬生长区盐沼沉积物微生物生物量、细菌丰度和群落组成的影响。于2013年7月、8月、9月和11月植物生长季节采集和分析经不同N量（尿素：0.1、0.2、0.4和0.8 g/kg（以干沉积物中N计））和不同N形式（尿素、（NH4）2SO4和NH4NO3各0.2 g/kg）1、2、3和4次处理后的沉积物及未经处理的对照样品。结果表明，N量和N形式对微生物生物量碳、微生物生物量氮和细菌丰度产生正影响。DGGE图谱显示，N量和N形式也影响细菌群落组成。研究表明，短期施氮能增加微生物生物量和细菌丰度，并且改变细菌群落结构。 |
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