| YI Xiaoyan,HUANG Yousong,ZHUANG Yunyun,CHEN Hongju,YANG Feifei,WANG Weimin,XU Donghui,LIU Guangxing,ZHANG Huan. 2017. In situ diet of the copepod Calanus sinicus in coastal waters of the South Yellow Sea and the Bohai Sea. Acta Oceanologica Sinica, 36(6):68－79 |
| In situ diet of the copepod Calanus sinicus in coastal waters of the South Yellow Sea and the Bohai Sea |
| 南黄海和渤海近海海域中华哲水蚤的现场摄食研究 |
| Received:March 21, 2016 Revised:August 02, 2016 |
| DOI：10.1007/s13131-017-0974-6 |
| Key words:copepod in situ diet molecular analysis 18S rDNA ciliate blocking primer |
| 中文关键词: 桡足类 现场摄食 分子分析 18S rDNA 纤毛虫阻断引物 |
| 基金项目:The National Natural Science Foundation of China under contract Nos 31372509, 41076085 and 41328009. |
| Author Name | Affiliation | E-mail | | YI Xiaoyan | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | HUANG Yousong | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | ZHUANG Yunyun | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | CHEN Hongju | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | YANG Feifei | College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | WANG Weimin | College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | XU Donghui | College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | LIU Guangxing | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | gxliu@ouc.edu.cn | | ZHANG Huan | Key Laboratory of Marine Environment and Ecology(Ocean University of China), Ministry of Education, Qingdao 266100, China
Department of Marine Sciences, University of Connecticut, Groton 06340, USA | |
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| Abstract: |
| Copepods are a key trophic link between primary producers and predatory animals at higher trophic levels in the marine ecosystem. Knowledge of the in situ composition of the copepod diet is critical for the accurate evaluation of trophic relationships and energy transfer in marine food webs. In this study, we applied a PCR-based cloning technique developed previously to investigate the in situ diet of Calanus sinicus, an ecologically important large-sized calanoid copepod that dominates in the shelf waters around China, Japan and Korea. Analyses of the 18S rDNA sequences obtained from the copepod diet revealed the diverse food composition of C. sinicus from two stations (Y19 in the South Yellow Sea and B49 in the Bohai Sea). A total of 43 operational taxonomic units (OTUs) were detected, which belonged to 13 diverse lineages:Bacillariophyta, Dinoflagellata, Dictyochophyceae, Chrysophyta, Katablepharidophyta, Pelagophyceae, Apusozoa, Hydrozoa, Ctenophora, Echinodermata, Tunicata, Chaetognatha and marine fungi. The results indicate that during an algae bloom, C. sinicus can graze on the bloom causative species. When the abundance of phytoplankton in ambient water is relatively low, C. sinicus can choose eggs, larvae, or organic particles/detritus of various metazoans, especially hydrozoans and ctenophores, as alternative food sources. Our result suggests that C. sinicus is an omnivorous species, and its prey choice may depend on the food availability in the ambient waters. |
| 中文摘要: |
| 桡足类是海洋生态系统中初级生产者和较高营养级消费者之间的关键联系环节，掌握桡足类的现场食物组成对于准确评估海洋食物网中的营养关系和能量转移至关重要。本研究中，我们以中华哲水蚤这一在中国、日本以及韩国近海具有重要生态地位的大型哲水蚤属桡足类为研究对象，应用之前开发的基于PCR的克隆技术，通过分析中华哲水蚤所摄食生物的18S rDNA序列，研究了中华哲水蚤的现场食物组成。结果揭示了南黄海（Y19站位）和渤海（B49站位）中华哲水蚤食物组成的多样性。共检测出43个操作分类单元（OTUs），分别隶属于13个类群：硅藻（Bacillariophyta）、甲藻（Dinoflagellata）、硅鞭藻（Dictyochophyceae）、金藻（Chrysophyta）、Katablepharidophyta、浮生藻（Pelagophyceae）、无根虫（Apusozoa）、水螅水母（Hydrozoa）、栉水母（Ctenophora）、棘皮动物（Echinodermata）、被囊动物（Tunicata）、毛颚动物（Chaetognatha）以及海洋真菌。结果还表明，当发生藻类暴发时，中华哲水蚤可以摄食引发藻类暴发的藻种。当周围海域浮游植物的丰度相对较低时，中华哲水蚤可以选择摄食各种后生动物尤其是水螅水母和栉水母的卵、幼虫或者有机碎屑。我们的研究结果表明中华哲水蚤是一种杂食性桡足类，它对食物的选择依赖其生活海域中食物的可获得性。 |
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