| QIAO Ying,WANG Jun,MAO Yong,LIU Min,SONG Xiaohong,SU Yongquan,WANG Chunzhong,ZHENG Zhipeng. 2017. Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus. Acta Oceanologica Sinica, 36(6):52－60 |
| Identification and molecular characterization of Cathepsin L gene and its expression analysis during early ontogenetic development of kuruma shrimp Marsupenaeus japonicus |
| 日本囊对虾Cathepsin L基因的鉴定及其在幼体发育中的表达研究 |
| Received:February 16, 2016 Revised:March 29, 2016 |
| DOI：10.1007/s13131-017-0983-5 |
| Key words:Cathepsin L gene larval development molting cycle tissue distribution Marsupenaeus japonicus |
| 中文关键词: 日本囊对虾 Mj-Cathepsin L 幼体发育 蜕皮阶段 组织表达 Marsupenaeus japonicus |
| 基金项目:The National High-tech R&D Program of China (863 Program) under contract No. 2012AA10A409-03; the Project of China Agriculture Research System under contract No. CARS-47; the Project of Xiamen Southern Ocean Research Center under contract No. 14CZY033HJ07; China Spark Program under contract No. 2015GA720002. |
| Author Name | Affiliation | E-mail | | QIAO Ying | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | | | WANG Jun | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | | | MAO Yong | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | | | LIU Min | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | | | SONG Xiaohong | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | | | SU Yongquan | State Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen 361102, China | yqsu@xmu.edu.cn | | WANG Chunzhong | Putian Tian-ran-xing Agricultural Development Co. Ltd., Fujian 351100, China | | | ZHENG Zhipeng | Putian Tian-ran-xing Agricultural Development Co. Ltd., Fujian 351100, China | |
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| Abstract: |
| Cathepsin L gene is a member of the cysteine proteinase gene group. In this study Cathepsin L gene was isolated from Kuruma shrimp Marsupenaeus japonicus (Mj-Cathepsin L) and the full-length DNA sequence was 1 963 bp. Mj-Cathepsin L protein showed high homologies with other Cathepsin L proteins documented in vertebrates, mollusks and other crustaceans. Expression analysis of Mj-Cathepsin L gene in different tissues revealed that it was predominant in hepatopancreas. During early ontogenetic development stages Mj-Cathepsin L showed a development-regulated expression, and the Mj-Cathepsin L showed a molting stage-regulated expression during the five molting stages, inferring its role in the ontogenic development of M. japonicus. Two kinds of forms of Mj-Cathepsin L protein:pro-Cathepsin L and Cathepsin L were measured in hepatopancreas, stomach and intestine by Western Blotting. |
| 中文摘要: |
| 本研究从日本囊对虾（Marsupenaeus japonica）中获得半胱氨酸蛋白酶家族中的cathepsin L基因，命名为Mj-Cathepsin L。Mj-Cathepsin L基因cDNA序列全长1191bp，其开放阅读框编码的Cathepsin L蛋白前体包含327个氨基酸，由信号肽，蛋白前体和成熟蛋白三部分组成。同源序列比对发现日本囊对虾Cathepsin L蛋白与其他已知物种的cahtepsin L蛋白具有较高的同源性。对Mj-Cathepsin L基因在不同组织以及早期幼体幼体发育阶段的研究发现，Mj-Cathepsin L主要在肝胰腺中大量表达，且在幼体发育不同阶段，其表达水平受发育阶段调控，且Mj-Cathepsin L基因显示出与其五个蜕皮时期相关的表达变化，说明Mj-Cathepsin L有可能在日本囊对虾个体发育过程中扮演不同的重要作用。并通过Western Blot方法在日本囊对虾肝胰腺，胃和肠中检测到Cathepsin L酶原和成熟Cathepsin L两种蛋白存在形式。 |
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