| YAO Jingyuan,LIU Yu,LI Ying,WANG Haixia. 2016. Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis. Acta Oceanologica Sinica, 35(6):1－6 |
| Effects of pretreatment procedures on fatty acid composition and stable carbon isotopes in the marine microalga Isochrysis zhanjiangenisis |
| 预处理方法对海洋微藻Isochrysis zhanjiangenisis脂肪酸组成及稳定碳同位素的影响 |
| Received:June 23, 2015 Revised:November 24, 2015 |
| DOI：10.1007/s13131-016-0837-6 |
| Key words:carbon stable isotope analysis fatty acid marine microalgae pretreatment |
| 中文关键词: 碳稳定同位素分析 脂肪酸 海洋微藻 预处理 |
| 基金项目: |
| Author Name | Affiliation | E-mail | | YAO Jingyuan | College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China | | | LIU Yu | College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China | ylsibo@gmail.com | | LI Ying | Environmental Information Institute, Dalian Maritime University, Dalian 116026, China | | | WANG Haixia | Environmental Information Institute, Dalian Maritime University, Dalian 116026, China | |
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| Abstract: |
| This study aims to quantify the effects of different pretreatment methods on the stable carbon isotope values of fatty acids in marine microalgae (Isochrysis zhanjiangenisis). To identify the effects of sample preparation on the δ13C value and the fatty acid composition, we examined eight types of pretreatment methods including: (a) drying the sample followed by direct methyl esterification using HCl-CH3OH; (b) drying the sample followed by direct methyl esterification using H2SO4-CH3OH; (c) drying the sample by ultrasonic extraction and methyl-esterification using HCl-CH3OH; (d) drying the sample by ultrasonic extraction and methyl-esterification using H2SO4-CH3OH; (e) fresh sample followed by direct methyl-esterification using HCl-CH3OH; (f) fresh sample followed by direct methyl-esterification using H2SO4-CH3OH; (g) fresh sample with ultrasonic extraction followed by methyl-esterification using HCl-CH3OH, and (h) fresh sample with ultrasonic extraction followed by methyl-esterification using H2SO4-CH3OH. The results show that the δ13C values from Groups a-e, g and h fluctuated within 0.3‰, and the δ13C values of Group f were approximately 0.7‰ lower than the other seven groups. Therefore, the different sample pretreatment methods used towards the extraction of fatty acids from marine microalgae may result in different results regarding the stable carbon isotope ratios, and if necessary a correction should be applied. |
| 中文摘要: |
| 本研究量化了不同预处理方法对海洋微藻(Isochrysis zhanjiangenisis)脂肪酸稳定碳同位素组成的影响。为确定样品预处理对微藻脂肪酸δ13C及脂肪酸含量的影响,采用八种预处理方法,包括:(a) 干燥样品,HCl-CH3OH直接甲酯化;(b) 干燥样品,H2SO4-CH3OH直接甲酯化;(c) 干燥样品,超声萃取后HCl-CH3OH甲酯化;(d) 干燥样品,超声萃取后H2SO4-CH3OH 甲酯化;(e) 新鲜样品,HCl-CH3OH直接甲酯化;(f) 新鲜样品,H2SO4-CH3OH直接甲酯化;(g) 新鲜样品,超声萃取后HCl-CH3OH甲酯化,以及 (h) 新鲜样品,超声萃取后H2SO4-CH3OH 甲酯化。结果表明:实验组 a-e, g 和 h中脂肪酸甲酯δ13C在0.3‰内波动,且实验组 f中脂肪酸甲酯δ13C低于其他组约0.7‰。因此,不同的海洋微藻预处理方法可能导致不同脂肪酸稳定碳同位素比,应给予适当修正。 |
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