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WANG Liping,ZHENG Binghui,LEI Kun. 2015. Diversity and distribution of bacterial community in the coastal sediments of Bohai Bay, China. Acta Oceanologica Sinica, 34(10):122-131
Diversity and distribution of bacterial community in the coastal sediments of Bohai Bay, China
Diversity and distribution of bacterial community in the coastal sediments of Bohai Bay, China
Received:June 17, 2014  Revised:September 25, 2014
DOI:10.1007/s13131-015-0719-3
Key words:Bohai Sea  coastal zone  aromatic hydrocarbon  bacteria  biodiversity  pyrosequencing
中文关键词:  Bohai Sea  coastal zone  aromatic hydrocarbon  bacteria  biodiversity  pyrosequencing
基金项目:The Central Basic Scientific Research Project in the Public Welfare for the Scientific Research Institutes under contract No. gyk5091301.
Author NameAffiliationE-mail
WANG Liping State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
State Environmental Protection Key Laboratory of Estuary and Coastal Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China 
wanglp@craes.org.cn 
ZHENG Binghui State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
State Environmental Protection Key Laboratory of Estuary and Coastal Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China 
 
LEI Kun State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
State Environmental Protection Key Laboratory of Estuary and Coastal Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China 
 
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Abstract:
      In order to understand the diversity and distribution of the bacterial community in the coastal sediment of the Bohai Bay, China, high-throughput barcoded pyrosequencing of the 16S rRNA gene was used. Metagenomic DNA was extracted from the sediment samples, and was sequenced using a 454 GS FLX Titanium system. At 97% similarity, the sequences were assigned to 22 884 operational taxonomic units (OTUs) which belonged to 41 phyla, 84 classes, 268 genera and 789 species. At the different taxonomic levels, both the dominants and their distribution varied significantly among the six coastal sediments. Proteobacteria was the first dominant phylum across all the six coastal sediments, representing 57.52%, 60.66%, 45.10%, 60.92%, 56.63% and 56.59%, respectively. Bacteroidetes was the second dominant phylum at Stas S1, S2 and S4, while Chloroflexi was the second dominant phylum at Stas S3, S5 and S6. At class level, γ-Proteobacteria was the first dominant class at Stas S1, S2, S4 and S6, while δ-Proteobacteria became the first dominant class at Stas S3 and S5. In addition, a large proportion of unclassified representatives have distributed at the different taxonomic levels. Canonical correspondence analysis (CCA) results indicated that the sediment texture, water depth (D), dissolved oxygen (DO), total nitrogen (TN) and nine EPA priority control polycyclic aromatic hydrocarbons (PAHs) including naphthalene, acenaphthylene, acenaphthene, fluorine, phenanthrene, fluoranthene, pyrene, benzo[a]anthracene and indeno[1,2,3-cd]pyrene were the important factors in regulating the bacterial community composition. Those results are very important to further understand the roles of bacterial community in the coastal biogeochemical cycles.
中文摘要:
      In order to understand the diversity and distribution of the bacterial community in the coastal sediment of the Bohai Bay, China, high-throughput barcoded pyrosequencing of the 16S rRNA gene was used. Metagenomic DNA was extracted from the sediment samples, and was sequenced using a 454 GS FLX Titanium system. At 97% similarity, the sequences were assigned to 22 884 operational taxonomic units (OTUs) which belonged to 41 phyla, 84 classes, 268 genera and 789 species. At the different taxonomic levels, both the dominants and their distribution varied significantly among the six coastal sediments. Proteobacteria was the first dominant phylum across all the six coastal sediments, representing 57.52%, 60.66%, 45.10%, 60.92%, 56.63% and 56.59%, respectively. Bacteroidetes was the second dominant phylum at Stas S1, S2 and S4, while Chloroflexi was the second dominant phylum at Stas S3, S5 and S6. At class level, γ-Proteobacteria was the first dominant class at Stas S1, S2, S4 and S6, while δ-Proteobacteria became the first dominant class at Stas S3 and S5. In addition, a large proportion of unclassified representatives have distributed at the different taxonomic levels. Canonical correspondence analysis (CCA) results indicated that the sediment texture, water depth (D), dissolved oxygen (DO), total nitrogen (TN) and nine EPA priority control polycyclic aromatic hydrocarbons (PAHs) including naphthalene, acenaphthylene, acenaphthene, fluorine, phenanthrene, fluoranthene, pyrene, benzo[a]anthracene and indeno[1,2,3-cd]pyrene were the important factors in regulating the bacterial community composition. Those results are very important to further understand the roles of bacterial community in the coastal biogeochemical cycles.
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