| ZENG Zhen,NI Jianbin,KE Caihuan. 2015. Glycogen content relative to expression of glycogen phosphorylase (GPH) and hexokinase (HK) during the reproductive cycle in the Fujian Oyster, Crassostrea angulata. Acta Oceanologica Sinica, 34(6):66－76 |
| Glycogen content relative to expression of glycogen phosphorylase (GPH) and hexokinase (HK) during the reproductive cycle in the Fujian Oyster, Crassostrea angulata |
| 糖原含量与糖原磷酸化酶基因(GPH)和己糖激酶基因(HK)的表达在福建牡蛎生殖周期中的相关性分析 |
| Received:February 20, 2014 Revised:July 04, 2014 |
| DOI：10.1007/s13131-015-0639-2 |
| Key words:Crassostrea angulate GPH HK glycogen metabolism reproduction |
| 中文关键词: 福建牡蛎 糖原磷酸化酶基因 己糖激酶基因 糖原代谢 生殖周期 |
| 基金项目:The National Basic Research Program (973 program) of China under contract No. 2010CB126403; the Program for Changjiang Scholars and Innovative Research Team of Xiamen University under contract No. IRT0941; the Earmarked Fund for Modern Agro-industry Technology Research System under contract No. nycytx-47; the Programme of Introducing Talents of Discipline to Universities under contract No. B07034. |
| Author Name | Affiliation | E-mail | | ZENG Zhen | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China | | | NI Jianbin | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China
National Marine Hazard Mitigation Service, Beijing 100194, China | | | KE Caihuan | State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China | chke@xmu.edu.cn |
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| Abstract: |
| Glycogen, a polymer of glucose, is an important means of storing energy. It is degraded by glycogen phosphorylase (GPH) and hexokinase (HK), glycogen phosphorylase, and hexokinase cDNAs (Ca-GPH and Ca-HK, respectively), which encode the primary enzymes involved in glycogen use, cloned and characterized and used to investigate the regulation of glycogen metabolism at the mRNA level in Crassostrea angulata. Their expression profiles were examined in different tissues and during different reproductive stages. Full-length cDNA of GPH was 3 078 bp in length with a 2 607 bp open reading frame (ORF) predicted to encode a protein of 868 amino acids (aa). The full-length HK cDNA was 3 088 bp long, with an ORF of 1 433 bp, predicted to encode a protein of 505 aa. Expression levels of both genes were found to be significantly higher in the gonads and adductor muscle than in the mantle, gill, and visceral mass. They were especially high in the adductor muscle, which suggested that these oysters can use glycogen to produce a readily available supply of glucose to support adductor muscle activity. The regulation of both genes was also found to be correlated with glycogen content via qRT-PCR and in situ hybridization and was dependent upon the stage of the reproductive cycle (initiation, maturation, ripeness). In this way, it appears that the expression of Ca-GPH and Ca-HK is driven by the reproductive cycle of the oyster, reflecting the central role played by glycogen in energy use and gametogenic development in C. angulata. It is here suggested that Ca-GPH and Ca-HK can be used as useful molecular markers for identifying the stages of glycogen metabolism and reproduction in C. angulata. |
| 中文摘要: |
| 糖原是重要的储能物质,它的降解由糖原磷酸化酶(GPH)和己糖激酶(HK)指导完成。而这两个酶分别由相应的基因GPH和HK指导合成。为了探讨福建牡蛎(Crassostrea angulata)在糖原代谢中相关基因的mRNA水平的变化,本研究克隆并分析了GPH和HK两个基因。其中GPH的cDNA全长为3078bp,具有2607bp的开放阅读框,预测编码868个氨基酸,而HK具有3088bp的全长,1433bp的开放阅读框,编码505个氨基酸。在本研究中,检测了两个基因在不同组织和生殖周期不同阶段的表达水平,发现两个基因均在性腺以及闭壳肌中呈现显著性的高表达,尤其在闭壳肌中表达量最高,推测与糖原释放和葡萄糖供给能力有密切相关。同时,这两个基因在原位杂交的实验中,也表现出与mRNA水平的变化一致。因此,研究推断,这两个基因受到福建牡蛎的生殖周期的调节,同时可以反映其在该周期中对能量释放的糖原代谢以及配子发育的具有相关性。所以,这两个基因具有作为福建牡蛎在糖原代谢以及生殖周期的分子标记潜在特性。 |
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