| ANDRIANTAHINA Farafidy,LIU Xiaolin,HUANG Hao. 2015. Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei. Acta Oceanologica Sinica, 34(6):59－65 |
| Using microsatellite markers to identify heritability of Pacific whiteleg shrimp Litopenaeus vannamei |
| 利用微卫星标记估计凡纳滨对虾生长性状的遗传力 |
| Received:January 15, 2014 Revised:May 16, 2014 |
| DOI：10.1007/s13131-015-0688-6 |
| Key words:genetic parameters growth heritability microsatellite molecular markers |
| 中文关键词: 遗传参数 生长 遗传力 微卫星 分子标记 |
| 基金项目:The Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China under contract No. 2012GB2E200361; the Key Laboratory of Marine Biology, Institute of Oceanlogy, Chinese Academy of Sciences; Qianjiang distinguished experts of Hangzhou; Extension and Demonstration Program in Desalting Culture of Litopenaeus Vannamei New Breed “Ke Hai 1”, Northwest A&F University, China under contract No. XNY2013-4. |
| Author Name | Affiliation | E-mail | | ANDRIANTAHINA Farafidy | College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China | | | LIU Xiaolin | College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China | liuxiaolin@nwsuaf.edu.cn | | HUANG Hao | Hainan Guangtai Ocean Breeding Company Limited, Haikou 570000, China | |
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| Abstract: |
| Pacific whiteleg shrimp (Litopenaeus vannamei) is an economically relevant shrimp species in many Asian countries. The specific objective of the current research was to assess microsatellite markers in screening the fastgrowth of domesticated L. vannamei stocks to establish a founder population for breeding-selection plans. The postlarvae produced by the reproduction of second generation broodstock were cultured in the same conditions throughout a five months growing period. Ninety juvenile shrimp were selected from the slow-, medium- and the fast-growth groups, and ten microsatellite markers were used to investigate their genetic diversity, and to understand the improvement of a breeding-selection scheme. Ten polymorphic loci (markers) (M1-M10) were produced at ten loci in this sample, among them Primer M8 was the highest polymorphic locus and M7 was the lowest one. A specific locus was found in the fast-growth group using Primer M5. The longest genetic distance (0.481) was determined between the fast- and medium-growth groups and the shortest (0.098) was between the slow- and medium-growth groups; therefore, the largest genetic identity (0.946) was observed between the slowand medium-growth groups and the smallest (0.667) was observed between the medium- and fast-growth groups. The Unweighted Paired Group with Arithmetic Average (UPGMA) dendrogram based on Nei's genetic distances provided two different groups; the first consist of the slow- and medium-growth groups and the second the fastgrowth group. Selection response and realized heritability for growth were 11.55% and 31.26%, respectively. Therefore, this set of microsatellite markers would provide a useful tool in shrimp breeding schemes. |
| 中文摘要: |
| 凡纳滨对虾(俗称南美白对虾)在许多亚洲国家是重要的对虾养殖种类。本研究的目的是筛选微卫星标记选择生长速度快的凡纳滨对虾养殖种群从而建立选育计划的基础群体。研究材料是由第二代亲虾繁殖产生的虾苗在相同条件下培养5个月的生长期对虾。从研究对象中分别选择90尾幼虾建成生长速度慢、生长速度中等、生长速度快的群体,10个微卫星标记用于研究遗传多样性,并研究选择育种的遗传进展。利用样品中筛选出10个多态性标记位点(M1-M10),其中,M8位点的多态性最高,M7的多态性最低,在快速生长组检测到多态性M5位点。快速生长组和中速生长组的遗传距离最大(0.481),中速生长组和慢速生长组之间遗传距离最短(0.098);因此,中速生长组和慢速生长组之间的遗传一致性最大(0.946),快速生长组和中速生长组之间的遗传一致性最小(0.667)。根据Nei的遗传距离和非加权配对组算术平均(UPGMA)聚类方法,将3个不同生长组聚类为两大类,第一类为中速生长组和慢速生长组,第二类为快速生长组。生长速度的选择反应和现实遗传力为分别增长11.55%和31.26%。因此,这套微卫星标记会在对虾育种方案提供了一个有用的工具。 |
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