| WANG Lei,HUANG Bangqin,LIU Xin,XIAO Wupeng. 2015. The modification and optimizing of the CHEMTAX running in the South China Sea. Acta Oceanologica Sinica, 34(2):124－131 |
| The modification and optimizing of the CHEMTAX running in the South China Sea |
| 浮游植物化学分类法—CHEMTAX运算在南中国海应用的改进和优化 |
| Received:October 02, 2013 Revised:February 24, 2014 |
| DOI：10.1007/s13131-015-0621-z |
| Key words:phytoplankton CHEMTAX pigment/Chl a ratio successive run South China Sea |
| 中文关键词: 浮游植物 CHEMTAX 色素/叶绿素a比值 迭代运算 南中国海 |
| 基金项目:The National Nature Science Foundation of China under contract Nos 40925018 and 41176112; the National Basic Research Program (973 Program) of China under contract No. 2009CB421203. |
| Author Name | Affiliation | E-mail | | WANG Lei | Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies / Key Laboratory of Coastal and Wetland Ecosystems of Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China | | | HUANG Bangqin | Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies / Key Laboratory of Coastal and Wetland Ecosystems of Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China | bqhuang@xmu.edu.cn | | LIU Xin | Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies / Key Laboratory of Coastal and Wetland Ecosystems of Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China | | | XIAO Wupeng | Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies / Key Laboratory of Coastal and Wetland Ecosystems of Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen 361102, China | |
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| Abstract: |
| In order to determine the phytoplankton community composition, the modification and optimizing of the CHEMical TAXonomy (CHEMTAX) running was carried out through samples grouping, successive run and evaluate the results for HPLC-pigment samples in the South China Sea (SCS). The vertical distribution of the ratio of pigment to total Chl a (TChl a) exhibited three different patterns, including increasing with depth pattern (e.g., But-Fuco), decreasing with depth pattern (e.g., Zea) and increasing at deep chlorophyll maximum (DCM) pattern (e.g., Hex-Fuco). The vertical profiles for Fuco/TChl a and Pras/TChl a was higher in coast than in the shelf and basin, and the Zea and Dv-Chl a expressed conversely. So the samples in the coastal stations must be separated for the cluster analysis group procedure in the SCS. Successive run was introduced into the CHEMTAX calculation and the output results were evaluated by the convergence of pigment/TChl a ratios. Most of the ratios were well converged at the fifth running, except Zea/TChl a for Prochlorococcus and Chl b/TChl a for prasinophytes and so on. To evaluate the fifth running's results, haptophytes_8 and chlorophytes were two phytoplankton groups with much uncertainty. But the fifth estimated value was better than running once was supported by the regression evidence between the measured pigment concentration and calculation values. Synechococcus was another component with much mutability, and the CHEMTAX's result should be compared to the flow cytometry's cell abundance. |
| 中文摘要: |
| 通过对南中国海基于浮游植物特征光合色素的化学分类法,即CHEMTAX程序因子分析方法进行本地化应用的调整和优化,本文探讨了计算中涉及的样品不同分组方式、迭代运算及结果评估等在CHEMTAX计算中的影响.结果表明,光合色素/总叶绿素a比值的垂直分布呈现出三种不同的类型:1.随深度增加而增大的类型,如But-Fuco等;2. 随深度增加而降低的类型,如Zea等;3. 随叶绿素最大层变化的类型,如Hex-Fuco等.在空间上来看,岩藻黄素与叶绿素a比值及青绿藻素与叶绿素a比值在近岸明显高于陆架和海盆区,而玉米黄素和二乙烯基叶绿素a则刚好相反,因此根据近岸区域色素比值的特殊性,在进行CHEMTAX运算时需要将近岸站位单独划分为一组.通过CHEMTAX迭代运算分析色素比值的收敛情况可以看出,大多数的色素/叶绿素a比值在第五次运算后都能较好收敛,但对于例如原绿球藻的玉米黄素与叶绿素a比值及青绿藻的叶绿素b与叶绿素a比值却并未能达到收敛.通过对实测值与计算值进行回归进而评估计算结果发现,定鞭藻 8型和绿藻类在计算中的不确定性相对较大,同时第五次迭代运算的结果要明显好于单独一次运算的结果.通过残差分析可以看出,聚球藻同样具有较大的不确定性,因而在今后的工作中应以光合色素配以流式细胞仪数据进行更为全面的生物量测定. |
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