| ZHANG Man,MAO Yong,WANG Jun,FENG Wenrong,SONG Xiaohong,SU Yongquan. 2015. Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus). Acta Oceanologica Sinica, 34(2):74－83 |
| Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp (Marsupenaeus japonicus) |
| 日本囊对虾一种具有双结构域的新型C-型凝集素的分子特性和表达分析 |
| Received:October 05, 2013 Revised:January 03, 2014 |
| DOI：10.1007/s13131-015-0623-x |
| Key words:C-type lectin Marsupenaeus japonicus carbohydrate-recognition domain (CRD) gene expression white spot syndrome virus (WSSV) |
| 中文关键词: C-型凝集素 日本囊对虾 糖识别结构域 基因表达 白斑综合征病毒 |
| 基金项目:The National High-Technology R&D Program (863 Program) of China under contract No. 2012AA10A409; China Agriculture Research System under contract No. CARS-47. |
| Author Name | Affiliation | E-mail | | ZHANG Man | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | MAO Yong | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | WANG Jun | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | FENG Wenrong | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | SONG Xiaohong | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | | | SU Yongquan | College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China | yqsu@xmu.edu.cn |
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| Abstract: |
| C-type lectins are among the most significant pattern recognition receptors (PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn (termed MjLectin) from kuruma shrimp Marsupenaeus japonicus was identified. The full-length cDNA of MjLectin was 1 245 bp with a 1 011 bp open reading frame (ORF) that encoded a polypeptide of 336 amino acid residues. MjLectin consisted of two tandemly arrayed carbohydrate-recognition domains (CRDs), unlike other reported M. japonicus C-type lectins with only one CRD. It showed a high similarity to other shrimp dual-CRD lectins. Among the Ca2+-binding Site 2, the tripeptide motif dictating the carbohydrate binding specificity was exhibited as a rare mutant LPN (Leu134-Pro135-Asn136) in CRD1 and a traditional EPN (Glu299-Pro300-Asn301) in CRD2, respectively. MjLectin showed a specific expression pattern in both tissue and cellular levels, for its mRNA transcript was mainly expressed in the F-cells of the hepatopancreas. After white spot syndrome virus (WSSV) challenge (3.6×108 virions/μL), the expression of MjLectin in the hepatopancreas was up-regulated significantly at 48 h (P<0.01) compared with the control group. These results suggested that MjLectin might be involved in the innate immune defense against WSSV infection. |
| 中文摘要: |
| C-型凝集素是无脊椎动物中一种重要的模式识别受体.它们是一类能够识别病原体表面特异性糖基的糖结合蛋白.本研究发现了一种新型的日本囊对虾C-型凝集素,命名为MjLectin.MjLectin cDNA 序列全长1245bp,开放阅读框长1011bp,编码336个氨基酸.不同于已报道的只含有一个糖识别结构域的日本囊对虾C-型凝集素,MjLectin具有两个串联排列的糖识别结构域.与其他含有两个结构域的对虾凝集素相比,MjLectin表现出较高的相似性.在两个结构域(CRD1和CRD2)的钙离子结合位点2中,决定糖结合特异性的三肽元件分别为突变的LPN (Leu134-Pro135-Asn136) 和保守的EPN (Glu299-Pro300-Asn301).MjLectin在组织和细胞水平上均具有表达特异性,主要在日本囊对虾肝胰腺的F细胞中表达.白斑综合征病毒(WSSV)攻毒后,肝胰腺中MjLectin的表达上调,并且在48小时含量极显著升高(P<0.01).以上结果表明,MjLectin可能参与了机体抵御WSSV感染的先天免疫防御. |
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