| XU Jingxiang,RUAN Lingwei,LI Zhen,YU Xiaoman,LI Sedong,SHI Hong,XU Xun. 2015. Characterization of four hemocyanin isoforms in Litopenaeus vannamei. Acta Oceanologica Sinica, 34(2):36－44 |
| Characterization of four hemocyanin isoforms in Litopenaeus vannamei |
| 凡纳滨对虾4种血蓝蛋白亚型的特征分析 |
| Received:November 04, 2013 Revised:February 27, 2014 |
| DOI：10.1007/s13131-015-0588-9 |
| Key words:hemocyanin SNPs isoforms Litopenaeus vannamei WSSV |
| 中文关键词: 血蓝蛋白 单核苷酸多态性 亚型 凡纳滨对虾 白斑综合症病毒 |
| 基金项目:The National High Technology Research and Development Program (863 Program) of China under contract No. 2012AA092205; the Major State Basic Research Development Program (973 Program) of China under contract No. 2012CB114403; the China Agriculture Research System-47; the National Natural Science Foundation of China under contract No. 31472297 and 31302209; the Scientific Research Foundation of Third Institute of Oceanography, SOA under contract No. 2011018. |
| Author Name | Affiliation | E-mail | | XU Jingxiang | School of Life Science, Xiamen University, Xiamen 361005, China
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | RUAN Lingwei | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | ruanlingwei@aliyun.com | | LI Zhen | Zhanjiang Evergreen South Ocean Science & Technology Company Limited, Zhanjiang 524094, China | | | YU Xiaoman | School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China | | | LI Sedong | Zhanjiang Ocean and Fishery Development Research Center, Zhanjiang 524000, China
Zhanjiang Evergreen South Ocean Science & Technology Company Limited, Zhanjiang 524094, China | | | SHI Hong | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | XU Xun | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | |
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| Abstract: |
| In this study, the gene encoding hemocyanin subunit L, LvHcL, was cloned from Litopenaeus vannamei and the genomic organization was characterized. This gene was diverse with many SNPs and also had at least four isoforms, while one of them (LvHcL4) only had two exons and the exon2 was missed. Transcription analysis showed that these isoforms of LvHcL were up-regulated after WSSV challenge in WSSV-resistant shrimp, while the transcriptions were decreased constantly in WSSV-susceptible shrimp. It is suggested that the hemocyanin had rich polymorphism and was involved in the antiviral response. These results could extend our previous findings and provide insights into the immune feature of hemocyanin, which would be helpful for further studies aimed at antiviral mechanism in inver-tebrate. |
| 中文摘要: |
| 血蓝蛋白是对虾体内的氧运输蛋白,占血浆中蛋白含量的95%以上.研究表明,它参与了许多生理功能,包括蛋白储运,渗透压调节,蜕皮过程以及骨架形成等等.近年来的研究表明,血蓝蛋白还参与了对虾的先天性免疫反应,具有酚氧化酶,抗菌肽、抗病毒等相关生物活性.本研究从凡纳滨对虾中克隆获得了血蓝蛋白L亚基LvHcL,并对其基因组织结构进行了分析.该基因具有丰富的多态性,含有多个SNP位点.研究中发现该基因亚基至少存在4种亚型,其中一种亚型缺失第二个外显子,仅含有2个外显子.对各个亚型的转录水平分析表明,抗病对虾在病毒感染刺激下,各个亚型的转录水平均会被诱导提高.而普通对虾在病毒感染刺激下,各个亚型的转录水平则被抑制.研究结果表明,血蓝蛋白参与了对虾的抗病毒免疫反应.这些结果不仅有助于深入了解对虾血蓝蛋白的免疫学特点,也有助于促进对无脊椎动物抗病毒免疫机制的认识. |
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