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Wu Yuehong,Cao Yi,Wang Chunsheng,Wu Min,Aharon Oren,Xu Xuewei. 2014. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge. Acta Oceanologica Sinica, 33(10):94-104
Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge
西南印度洋深海热液硫化物区沉积物微生物群落结构和固氮基因多样性
Received:March 25, 2013  Revised:November 02, 2013
DOI:10.1007/s13131-014-0544-0
Key words:deep-sea  hydrothermal vent  microbial diversity  16S rRNA gene  nifH gene
中文关键词:  深海  热液喷口  微生物多样性  16S rRNA  nifH
基金项目:The China Ocean Mineral Resources R & D Association (COMRA) Special Foundation under contract No. DY125-15-R-03; the National Natural Science Foundation of China under contract Nos 41276173 and 41206104; the Scientific Research Fund of the Second Institute of Oceanography, State Oceanic Administration under contract No. JT1011.
Author NameAffiliationE-mail
Wu Yuehong Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China  
Cao Yi State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China  
Wang Chunsheng Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China  
Wu Min College of Life Sciences, Zhejiang University, Hangzhou 310058, China  
Aharon Oren Department of Plant and Environmental Sciences, the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
The Hebrew University of Jerusalem, The Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel 
 
Xu Xuewei Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China xuxw@sio.org.cn 
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Abstract:
      A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilon Proteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the Southwest Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III nifH were present. This is consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.
中文摘要:
      沉积物样品来自西南印度洋深海热液硫化物区,水深为2951米。通过扩增其16S rRNA 和nifH基因研究微生物群落结构。古菌克隆主要为MBGE(Marine Benthic Group E)和MGI (Marine GroupⅠ)类群,分别属于广古菌门和奇古菌门。超过一半的细菌克隆属于变形杆菌(Proteobacteria),且大部分为γ-变形菌纲。未检测到ε-变形杆菌纲的克隆。此外还检测到放线菌门、拟杆菌门、浮霉菌门、酸杆菌门、消化螺旋菌门、绿弯菌门、绿菌门、衣原体门、疣微菌门和几个候补类群(OD1、 OP11、WS3、TM6),表明这些类群存在于热液硫化物环境。固氮基因(nifH)研究揭示了热液硫化物环境存在生物氮固定过程。系统发育分析表明该环境只存在类群I和类群III固氮基因。这和16S rRNA系统发育分析结果一致,表明细菌在热液硫化物环境氮固定过程中可能发挥了主要作用。
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