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Huang Yousong,Liu Guangxing,Chen Xiaofeng. 2014. Molecular phylogeography and population genetic structure of the planktonic copepod Calanus sinicus Brodsky in the coastal waters of China. Acta Oceanologica Sinica, 33(10):74-84
Molecular phylogeography and population genetic structure of the planktonic copepod Calanus sinicus Brodsky in the coastal waters of China
中国近海浮游桡足类中华哲水蚤(Calanussinicus Brodsky)的分子系统地理学和种群遗传结构研究
Received:April 13, 2013  Revised:December 17, 2013
DOI:10.1007/s13131-014-0542-2
Key words:copepod  Calanus sinicus  mtCOI  population genetic differentiation  China Coastal Current
中文关键词:  桡足类  中华哲水蚤  线粒体细胞色素氧化酶亚基I基因  种群遗传分化  中国沿岸流
基金项目:The National Natural Science Foundation of China under contract Nos 40876066 and 41076085; the National Basic Research Program of China under contract No. 2005CB422306.
Author NameAffiliationE-mail
Huang Yousong College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China  
Liu Guangxing College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
Key Laboratory of Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao 266100, China 
gxliu@ouc.edu.cn 
Chen Xiaofeng College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China  
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Abstract:
      Planktonic copepod Calanus sinicus is the dominant meso-zooplankton in the Northwest Pacific Ocean. To better understand its population dynamics and phylogeographic patterns, 243 C. sinicus individuals were collected from seven locations across the shelf waters of China and its population genetics was studied by mitochondrial DNA cytochrome oxidase I (mtCOI) sequences analyses. Thirty-nine different sequences, or haplotypes, were detected with moderate haplotype diversity (h=0.749) and low nucleotide diversity (π=0.003) for all populations. The evolutionary divergence between geographic populations varied from 0.24% to 0.37%, indicative of very limited genetic differentiation. Visualized minimum spanning network (MSN) and phylogenetic analysis of all the detected haplotypes did not reveal any clear phylogeographic pattern. Furthermore, AMOVA data showed no significant spatial population differentiation existed among the individuals collected across China shelf waters. Pairwise FST values showed that population collected from northwest of the East China Sea (ECS) displayed a low difference to other populations. Mismatch distribution analyses and neutrality tests indicated that C. sinicus might undergo a demographic/population expansion. No significant population genetic structuring was detected, indicating an extensive gene flow among the C. sinicus populations. Our results provide molecular evidence for the hypothesis that C. sinicus in the northwestern South China Sea in winter is transported from the East China Sea and the Yellow Sea by the China Coastal Current during the northeast monsoon period.
中文摘要:
      中华哲水蚤(Calanussinicus)是西北太平洋浮游动物中的优势种,在中国近海生态系统中具有重要的生态地位。为了分析中华哲水蚤种群遗传结构和遗传多样性,更好地理解其种群动态和系统地理格局,我们提取了中国近海7个站位的243个中华哲水蚤个体的基因组DNA。基于线粒体细胞色素氧化酶亚基I基因(mtCOI)的分析,共发现39种单倍型序列,单倍型多样性(h)为0.749,核苷酸多样性(π)为0.003。中华哲水蚤不同地理群体之间的遗传距离为0.24%~0.37%,表明不同地理群体之间的遗传分化非常有限。单倍型最小网络跨度树(Minimum Spanning Network,MSN)和系统发育分析显示,不同单倍型的分布没有明显的地理分化。分子方差分析(AMOVA)表明中国近岸水域的中华哲水蚤不同地理群体之间没有明显的分化。FST分析显示东海西北部群体(ECS)与其它地理群体间有程度较低的差异。核苷酸不配对分析(Mismatch distribution analysis)和中性检验(Neutrality tests)表明中华哲水蚤可能正在经历种群扩张。对所有不同地理群体的中华哲水蚤而言,没有检测到明显的种群遗传结构,表明中国近海中华哲水蚤各地理群体之间有着广泛的基因交流。另外,我们的研究结果为黄东海的中华哲水蚤在东北季风时节由中国沿岸流携带进入南海西北部的假设提供了分子水平上的证据。
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