| CHI Shan,QIAN Hao,LI Tianyong,WANG Xumin,LIU Cui,REN Lei,TANG Xuexi,LIU Tao. 2014. Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers. Acta Oceanologica Sinica, 33(9):139－151 |
| Phylogeny of genera Laminaria and Saccharina (Laminariales, Phaeophyceae) based on three molecular markers |
| 褐藻纲海带目Laminaria属和Saccharina属基于三个分子标记的系统发育分析 |
| Received:February 07, 2013 Revised:February 11, 2014 |
| DOI：10.1007/s13131-014-0525-3 |
| Key words:Saccharina Laminaria phylogeny COI rbcL ITS |
| 中文关键词: Saccharina Laminaria 系统进化 COI rbcL ITS |
| 基金项目:The National Natural Science Foundation of China under contract No. 41206116; the Postdoctoral Science Foundation of China under contract No. 2011M501167; the National Public Benefit Research Foundation of China under contract No. 201105021; the Fundamental Research Funds for the Central Universities under contract No. 201362040. |
| Author Name | Affiliation | E-mail | | CHI Shan | Ocean University of China, Qingdao 266003, China | | | QIAN Hao | Ocean University of China, Qingdao 266003, China | | | LI Tianyong | Ocean University of China, Qingdao 266003, China | | | WANG Xumin | Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
Chinese Academy of Sciences Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China | | | LIU Cui | Ocean University of China, Qingdao 266003, China | liutao@ouc.edu.cn | | REN Lei | Ocean University of China, Qingdao 266003, China | | | TANG Xuexi | Ocean University of China, Qingdao 266003, China | | | LIU Tao | Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| In the past two decades, many studies have focused on the classification within genus Laminaria, ultimately trying to divide it into two subgroups or genera: Laminaria and Saccharina. A significant debate still surrounds the question of its division, as the conflicting phylogenetic hypotheses that have resulted from the classification studies are based on different taxon sampling, molecular markers, or analysis methods. It is aimed at elucidate the molecular phylogeny within Laminaria and Saccharina. The nine species of Laminariales are sampled from northern Asia and Europe, and 23 new sequences in the nuclear, plastid, and mitochondrial genomes are determined to identify their taxonomic status. The phylogenetic analyses of 71 species are performed, including representatives from six of the seven families of the order Laminariales, based on three separate data sets. An evidence is provided to strongly support a clear split that maintains the two recognized genera, Laminaria and Saccharina, with Laminaria appearing to be the ancestor group. Further, analyses indicate that all taxa in Saccharina and Laminaria did not form a monophyletic lineage, instead Laminariaceae and Lessoniaceae grouped together interlacedly, and Costariaceae appeared as the sister taxon of the Lessoniaceae-Laminariaceae clade. In the phylogenetic analysis, mitochondrial c oxidase I (COI) sequences appeared to be the most credible molecular marker which was more befitting than nuclear encoded internal transcribed spacer (ITS) and plastid encoded rbcL for establishment of Laminariales systematics. It is the most comprehensive phylogeny of the order Laminariales, and contributes to an enhanced understanding and estimation of the phylogenetic relationships for the economically important seaweeds, Laminaria and Saccharina. |
| 中文摘要: |
| 在过去的二十年中，在海带目Laminaria属的分类鉴定方面存在着很多假说，目前趋向于将其划分为两个属或亚组：Laminaria和Saccharina。然而这种划分方式仍然存在着争论，原因在于不同的取样类群、分子标记选择、或分析方法的差异产生了不一致的系统发育假说。为了明确阐述Laminaria和Saccharina的系统发育关系，我们从亚洲北部和欧洲对海带目9个物种进行了采样，扩增获得了23条来源于核基因组、质体基因组和线粒体基因组的新序列。基于核编码ITS序列、质体编码rbcL和线粒体编码COI序列，进行了包括海带目6个科71个物种的系统进化分析。研究证明Laminaria和Saccharina分成了两个独立分支，而Laminaria分支为原始类群。此外，分析表明Laminaria和Saccharina中物种形成非单系群，和Lessoniaceae科物种聚类在一起，而Costariaceae科为其姐妹群。在海带目的系统发育关系研究方面，相较而言COI序列可以作为比较可靠的分子标记物。本论文是目前囊括物种数目最多的海带目系统进化的研究，有助于提高我们对于重要经济海藻海带属物种的进化地位以及亲缘关系的认识。 |
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