| ZHAO Xiaobo,PANG Shaojun,LIU Feng,SHAN Tifeng,LI Jing. 2014. Biological identification and determination of optimum growth conditions for four species of Navicula. Acta Oceanologica Sinica, 33(8):111－118 |
| Biological identification and determination of optimum growth conditions for four species of Navicula |
| Biological identification and determination of optimum growth conditions for four species of Navicula |
| Received:November 14, 2012 Revised:June 17, 2013 |
| DOI：10.1007/s13131-014-0465-y |
| Key words:Navicula SSU rbcL culture conditions |
| 中文关键词: Navicula SSU rbcL culture conditions |
| 基金项目:The National Natural Science Foundation of China under contract No. 41176135. |
| Author Name | Affiliation | E-mail | | ZHAO Xiaobo | Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | PANG Shaojun | Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | sjpang@qdio.ac.cn | | LIU Feng | Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | SHAN Tifeng | Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | LI Jing | Algal Culture Collection Centre, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
University of Chinese Academy of Sciences, Beijing 100049, China | |
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| Abstract: |
| Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy (SEM) and sequence comparisons of two segments of genes (small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm (OD680) under various environmental factors. Results showed that the optimum culture conditions were 25℃, 50–100 μmol photons m-2 s-1, pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates (RGR) peaked at the highest tested level (0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration. |
| 中文摘要: |
| Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy (SEM) and sequence comparisons of two segments of genes (small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm (OD680) under various environmental factors. Results showed that the optimum culture conditions were 25℃, 50–100 μmol photons m-2 s-1, pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates (RGR) peaked at the highest tested level (0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration. |
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