| WANG Xiuxiu,HUANG Bangqin,ZHANG Huan. 2014. Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii. Acta Oceanologica Sinica, 33(4):85－91 |
| Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii |
| Phosphorus deficiency affects multiple macromolecular biosynthesis pathways of Thalassiosira weissflogii |
| Received:September 23, 2012 Revised:December 31, 2012 |
| DOI：10.1007/s13131-014-0413-x |
| Key words:phosphorus deficiency Thalassiosira weissflogii LFQ-LC-MS/MS sulfolipid biosynthesis protein |
| 中文关键词: phosphorus deficiency Thalassiosira weissflogii LFQ-LC-MS/MS sulfolipid biosynthesis protein |
| 基金项目:The National Natural Science Foundation of China (NSFC) under contract No. 40925018; the National Basic Research Program (973 Program) under contract No. 2011CB403603. |
| Author Name | Affiliation | E-mail | | WANG Xiuxiu | Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, Xiamen University, Xiamen 361005, China
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | | | HUANG Bangqin | Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem, Xiamen University, Xiamen 361005, China
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China | bqhuang@xmu.edu.cn | | ZHANG Huan | Department of Marine Sciences, University of Connecticut, Groton 06340, USA | |
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| Abstract: |
| Phosphorus (P) is one of the key nutrients for the growth of phytoplankton. In this study, we used a method coupling label-free quantitation with liquid chromatography-mass spectrometry (LFQ-LC-MS/MS) to track the change of relative protein abundance between P-replete and P-deficient treatments in a non-model diatom, Thalassiosira weissflogii. Out of the 631 proteins identified, 132 were found to have significant changes in abundance (>1.5 folds) between the two treatments, especially those proteins involved in macromolecular biosynthesis pathways. For example, the up-regulation of sulfolipid biosynthesis protein in the P-deficient culture suggested a switch from using phospholipids to sulfolipids. In addition, the ribosome subunits and tRNA synthetases were down-regulated, which might explain the decrease in protein content in the P-deficient culture. A vacuolar sorting receptor homologous protein was found to be 9.2-folds up-regulated under P-deficiency, indicating an enhancement in the vacuolar sorting pathway for protein degradation. Our results show that T. weissflogii has sophisticated responses in multiple macromolecular metabolism pathways under P-deficiency, a mechanism which can be critical for this species to survive under various levels of P availability in the environment. |
| 中文摘要: |
| Phosphorus (P) is one of the key nutrients for the growth of phytoplankton. In this study, we used a method coupling label-free quantitation with liquid chromatography-mass spectrometry (LFQ-LC-MS/MS) to track the change of relative protein abundance between P-replete and P-deficient treatments in a non-model diatom, Thalassiosira weissflogii. Out of the 631 proteins identified, 132 were found to have significant changes in abundance (>1.5 folds) between the two treatments, especially those proteins involved in macromolecular biosynthesis pathways. For example, the up-regulation of sulfolipid biosynthesis protein in the P-deficient culture suggested a switch from using phospholipids to sulfolipids. In addition, the ribosome subunits and tRNA synthetases were down-regulated, which might explain the decrease in protein content in the P-deficient culture. A vacuolar sorting receptor homologous protein was found to be 9.2-folds up-regulated under P-deficiency, indicating an enhancement in the vacuolar sorting pathway for protein degradation. Our results show that T. weissflogii has sophisticated responses in multiple macromolecular metabolism pathways under P-deficiency, a mechanism which can be critical for this species to survive under various levels of P availability in the environment. |
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