| FENG Yanjing,CHI Shan,LIU Cui,CHEN Shengping,YU Jun,WANG Xumin,LIU Tao. 2014. The discovery of archaea origin phosphomannomutase in algae based on the algal transcriptome. Acta Oceanologica Sinica, 33(2):108－113 |
| The discovery of archaea origin phosphomannomutase in algae based on the algal transcriptome |
| The discovery of archaea origin phosphomannomutase in algae based on the algal transcriptome |
| Received:March 27, 2013 Revised:July 30, 2013 |
| DOI：10.1007/s13131-014-0447-0 |
| Key words:transcriptomic sequencing Chromista phosphomannomutase conserved motif phylogenetic analysis |
| 中文关键词: transcriptomic sequencing Chromista phosphomannomutase conserved motif phylogenetic analysis |
| 基金项目:The National High Technology Research and Development Program of China under contract No.2012AA10A406; the National Natural Science Foundation of China under contract Nos 41206116, 31140070 and 31271397; Technology Project of Ocean and Fisheries of Guangdong Province under contract No. A201201E03; the Fundamental Research Funds for the Central Universities under contract No. 201262003; China Postdoctoral Science Foundation under contract No. 2011M501167; the algal transcriptome sequencing was supported by 1KP Project (www.onekp.com). |
| Author Name | Affiliation | E-mail | | FENG Yanjing | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | CHI Shan | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | LIU Cui | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | CHEN Shengping | Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism, Zhong Shan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China | | | YU Jun | CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China | | | WANG Xumin | CAS Key Laboratory of Genome Sciences and Information, Beijing Key Laboratory of Genome and Precision Medicine Technologies, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
Beijing Key Laboratory of Functional Genomics for Dao-di Herbs, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China | wangxm@big.ac.cn | | LIU Tao | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | liutao@ouc.edu.cn |
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| Abstract: |
| phosphomannomutase (PMM; EC 5.4.2.8) is an enzyme that catalyzes the interconversion reaction between mannose-6-phosphate and mannose-1-phosphate. However, its systematic molecular and functional investigations in algae have not hitherto been reported. In this work, with the accomplishment of the 1 000 Plant Project (OneKP) in which more than 218 species of Chromista, including 19 marine phaeophytes, 22 marine rhodophytes, 171 chlorophytes, 5 cryptophytes, 4 haptophytes, and 5 glaucophytes were sequenced, we used a gene analysis method to analyze the PMM gene sequences in algae and confirm the existence of the PMM gene in the transcriptomic sequencing data of Rhodophyta and Ochrophyta. Our results showed that only one type of PMM with four conserved motifs exists in Chromista which is similar to human PMM. Moreover, the phylogenetic tree revealed that algae PMM possibly originated from archaea. |
| 中文摘要: |
| phosphomannomutase (PMM; EC 5.4.2.8) is an enzyme that catalyzes the interconversion reaction between mannose-6-phosphate and mannose-1-phosphate. However, its systematic molecular and functional investigations in algae have not hitherto been reported. In this work, with the accomplishment of the 1 000 Plant Project (OneKP) in which more than 218 species of Chromista, including 19 marine phaeophytes, 22 marine rhodophytes, 171 chlorophytes, 5 cryptophytes, 4 haptophytes, and 5 glaucophytes were sequenced, we used a gene analysis method to analyze the PMM gene sequences in algae and confirm the existence of the PMM gene in the transcriptomic sequencing data of Rhodophyta and Ochrophyta. Our results showed that only one type of PMM with four conserved motifs exists in Chromista which is similar to human PMM. Moreover, the phylogenetic tree revealed that algae PMM possibly originated from archaea. |
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