| KONG Fanna,ZHOU Yang,SUN Peipei,LIU Limin,MAO Yunxiang. 2013. Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina. Acta Oceanologica Sinica, 32(8):68－78 |
| Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species, Zostera marina |
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| Received:March 22, 2012 Revised:February 28, 2013 |
| DOI：10.1007/s13131-013-0343-z |
| Key words:expressed sequence tags (ESTs) gene function,metallothionein salt-tolerant genes Zostera marina |
| 中文关键词: |
| 基金项目:The Key Science and Technology Programof Shandong Province under contract No. 2012GHY11527; Natural Science Foundation of Shandong Province under contract No. Q2007E02; Specialized Research Fund for the Doctoral Program of Higher Education (New Teachers) under contract No. 20070423027; the Public Science and Technology Research Funds Projects ofOcean, StateOceanic Administration of the People's Republic of China under contract No. 201105021-8. |
| Author Name | Affiliation | E-mail | | KONG Fanna | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China | fnkong@ouc.edu.cn | | ZHOU Yang | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China | | | SUN Peipei | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China | | | LIU Limin | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China | | | MAO Yunxiang | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Key Laboratory of Marine Biology Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China | |
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| Abstract: |
| Zosteramarina, a monocotyledonous angiosperm, is one of themost important seagrass species. To investigate the salt-tolerancemechanismand discover salt-tolerant genes in Z. marina, a cDNA library was constructed. Single-pass sequencing of the 5' ends of 4 081 clones yielded 4 002 high quality expressed sequence tags (ESTs), which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from100-1 500 bp. Basic Local Alignment Search Tool (BLASTX) analysis revealed that 1 664 unigenes had significant homology to known genes in the National Center for Biotechnology Information (NCBI) non-redundant (nr) database (E-value≤5-10). Among them, the twomost abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes (KEGG), 1 462 unigeneswere assigned to 1 161 pathways (E-value≤5-10). A total of 938 unigenes were assigned Gene Ontology (GO) terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes formetallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z.marina. |
| 中文摘要: |
| Zosteramarina, a monocotyledonous angiosperm, is one of themost important seagrass species. To investigate the salt-tolerancemechanismand discover salt-tolerant genes in Z. marina, a cDNA library was constructed. Single-pass sequencing of the 5' ends of 4 081 clones yielded 4 002 high quality expressed sequence tags (ESTs), which were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. The average length of the ESTs was 582 bp, with sizes ranging from100-1 500 bp. Basic Local Alignment Search Tool (BLASTX) analysis revealed that 1 664 unigenes had significant homology to known genes in the National Center for Biotechnology Information (NCBI) non-redundant (nr) database (E-value≤5-10). Among them, the twomost abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), accounting for 7.1% and 1.7% of the total ESTs, respectively. Using Kyoto Encyclopedia of Genes and Genomes (KEGG), 1 462 unigeneswere assigned to 1 161 pathways (E-value≤5-10). A total of 938 unigenes were assigned Gene Ontology (GO) terms based on the GO hierarchy analysis, and InterProScan searches recognized 1 003 InterPro families. Three genes formetallothionein in Z. marina that belonged to Class II was identified. Results of this study will improve understanding of the molecular mechanisms of saline tolerance in Z.marina. |
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