| QIN Yingxue,YAN Qingpi,SU Yongquan,LI Haiping,ZOU Wenzheng. 2013. Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi. Acta Oceanologica Sinica, 32(8):55－60 |
| Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi |
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| Received:April 09, 2012 Revised:December 07, 2012 |
| DOI：10.1007/s13131-013-0341-1 |
| Key words:chemotactic motility Vibrio harveyi transposon virulence |
| 中文关键词: |
| 基金项目:The National Natural Science Foundation of China under contract Nos 31272699 and 41176115; National Department Public Benefit Research Foundation of China under contract No. 200903029; the Natural Science Foundation of Fujian Province under contract No. 2011J06014; the National Hi-Tech Research and Development Programof China (863 Program) under contract No. 2007AA09Z115. |
| Author Name | Affiliation | E-mail | | QIN Yingxue | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China
Fisheries College, Jimei University, Xiamen 361021, China | | | YAN Qingpi | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China
Fisheries College, Jimei University, Xiamen 361021, China | yanqp@jmu.edu.cn | | SU Yongquan | College of Ocean and Earth Science, Xiamen University, Xiamen 361005, China | | | LI Haiping | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China
Fisheries College, Jimei University, Xiamen 361021, China | | | ZOU Wenzheng | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China
Fisheries College, Jimei University, Xiamen 361021, China | |
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| Abstract: |
| Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi. |
| 中文摘要: |
| Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (GenBank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (GenBank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth,motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energymetabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotacticmotility of V. harveyi. |
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