| SHAO Changwei,LIU Geng,LIU Shanshan,LIU Changlin,CHEN Songlin. 2013. Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Acta Oceanologica Sinica, 32(8):35－43 |
| Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts |
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| Received:June 11, 2012 Revised:December 22, 2012 |
| DOI：10.1007/s13131-013-0339-8 |
| Key words:BAC cyp19a1a conservation gene synteny half-smooth tongue sole |
| 中文关键词: |
| 基金项目:The National Nature Science Foundation of China under contract Nos 41006107, 31072202 and 31130057; the National High- Technology R&D Programof China (863 Program) under contract No. 2012AA10A403-2; the Special Fund for Agro-scientific Research in the Public Interest of China under contract No. 200903046; Taishan Scholar Project Fund of Shandong of China. |
| Author Name | Affiliation | E-mail | | SHAO Changwei | Ocean University of China, Qingdao 266071, China
Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | | | LIU Geng | BGI-Shenzhen, Shenzhen 518000, China | | | LIU Shanshan | Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | | | LIU Changlin | Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | | | CHEN Songlin | Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, China | chensl@ysfri.ac.cn |
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| Abstract: |
| The cyp19a1a gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome (BAC) sequence of half-smooth tongue sole (Cynoglossus semilaevis) containing the intact cyp19a1a gene was reported and the conservation and synteny of the cyp19a1a gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine (GC) content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19a1a gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The expression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19a1a genes not only have similar genomic structures, but also have conserved functions. The genomic environment of cyp19a1a in tongue sole is not universal in teleosts, reflecting the particular evolution of tongue sole cyp19a1a after it diverged from the other teleosts. |
| 中文摘要: |
| The cyp19a1a gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome (BAC) sequence of half-smooth tongue sole (Cynoglossus semilaevis) containing the intact cyp19a1a gene was reported and the conservation and synteny of the cyp19a1a gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine (GC) content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19a1a gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The expression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19a1a genes not only have similar genomic structures, but also have conserved functions. The genomic environment of cyp19a1a in tongue sole is not universal in teleosts, reflecting the particular evolution of tongue sole cyp19a1a after it diverged from the other teleosts. |
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