| LIU Yun,SONG Xiuxian,HAN Xiaofian,YU Zhiming. 2013. Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum. Acta Oceanologica Sinica, 32(6):82－88 |
| Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum |
| Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum |
| Received:March 23, 2012 Revised:September 12, 2012 |
| DOI：10.1007/s13131-013-0324-2 |
| Key words:high-affinity nitrate transporter gene phosphate starvation nitrate concentrations real-time PCR Skeletonema costatum |
| 中文关键词: high-affinity nitrate transporter gene phosphate starvation nitrate concentrations real-time PCR Skeletonema costatum |
| 基金项目:The National Basic Research Program of China (973 Program) under contract No.2010CB428706; the National Natural Science Foundation of China for Creative Research Groups under contract No.41121064; the National High Technology Research and Development Program of China under contract No.2008AA09Z107. |
| Author Name | Affiliation | E-mail | | LIU Yun | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate School of the Chinese Academy of Sciences, Beijing 100049, China | | | SONG Xiuxian | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | HAN Xiaofian | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | YU Zhiming | Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | zyu@qdio.ac.cn |
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| Abstract: |
| To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built:y=-3.305x+98.95, R2=0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels. |
| 中文摘要: |
| To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 μmol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built:y=-3.305x+98.95, R2=0.988, where x represents nitrate concentrations (<40 μmol/L) and y represents the Nrt2 transcript levels. |
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