| LIU Lupeng,WANG Fengping,XU Jun,XIAO Xiang. 2013. Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents. Acta Oceanologica Sinica, 32(6):75－81 |
| Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents |
| Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents |
| Received:April 11, 2012 Revised:October 28, 2012 |
| DOI：10.1007/s13131-013-0323-3 |
| Key words:hydrothermal vents Thermococcales Guaymas Basin |
| 中文关键词: hydrothermal vents Thermococcales Guaymas Basin |
| 基金项目:The National Basic Research Program of China under contract No. 2011CB808800; the National Natural Science Foundation of China under contract No. 40830213; the Science and Technology Commission of Shanghai Municipality under contract No. 10]C1406700; the State Key Laboratory of Ocean Engineering in China under contract No. GI(ZD010053-1; US-National Science Foundation under contract No. OCE-0728391. |
| Author Name | Affiliation | E-mail | | LIU Lupeng | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology
State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | WANG Fengping | State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology
State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
School of System Biology, Shanghai Jiao Tong University, Shanghai 200240, China | | | XU Jun | State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology
State Key Laboratory of Ocean Engineering, Shanghai Jiao Tong University, Shanghai 200240, China | | | XIAO Xiang | State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology | xoxiang@sjtu.edu.cn |
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| Abstract: |
| Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrothermal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the strains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyrococcus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demonstrates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment. |
| 中文摘要: |
| Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrothermal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the strains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyrococcus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demonstrates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment. |
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