| CHEN Xiao,AI Weiming,YE Le,WANG Xuehui,LIN Chongwen,YANG Shengyun. 2013. The complete mitochondrial genome of the grey bamboo shark (Chiloscyllium griseum) (Orectolobiformes: Hemiscylliidae): genomic characterization and phylogenetic application. Acta Oceanologica Sinica, 32(4):59－65 |
| The complete mitochondrial genome of the grey bamboo shark (Chiloscyllium griseum) (Orectolobiformes: Hemiscylliidae): genomic characterization and phylogenetic application |
| The complete mitochondrial genome of the grey bamboo shark (Chiloscyllium griseum) (Orectolobiformes: Hemiscylliidae): genomic characterization and phylogenetic application |
| Received:February 14, 2012 Revised:June 20, 2012 |
| DOI：10.1007/s13131-013-0298-0 |
| Key words:mitochondrial genome Chiloscyllium griseum Chondrichthyes phylogeny carpet shark Galeomorphii |
| 中文关键词: mitochondrial genome Chiloscyllium griseum Chondrichthyes phylogeny carpet shark Galeomorphii |
| 基金项目:The National Natural Science Foundation of China under contract No. 41006080; Ocean Public Welfare Scientific Research Project under contract Nos 200905019 and 201105008-4; Science and Technology Department of Zhejiang Province, China under contract No. 2007F10011. |
| Author Name | Affiliation | E-mail | | CHEN Xiao | College of Fisheries, Ocean University of China, Qingdao 266003, China
Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, China | | | AI Weiming | Department of Marine Science, School of Life Science, Wenzhou Medical College, Wenzhou 325035, China | | | YE Le | South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China | | | WANG Xuehui | South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China | | | LIN Chongwen | Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, China | | | YANG Shengyun | Laboratory of Marine Biodiversity and Global Change, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China | Yangsy@xmu.edu.cn |
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| Abstract: |
| The complete mitochondrial genome of the grey bamboo shark, Chiloscyllium griseum (Orectolobiformes:Hemiscylliidae) was obtained, with a total of 16 755 base pairs (bp). The mitogenome encoded 13 proteincoding genes, two ribosomal RNAs, 22 transfer RNAs and a noncoding control region, a gene arrangement identical to the most common type found in vertebrates. All tRNA sequences were folded into typical cloverleaf secondary structure with the exception of tRNA-Ser2, in which the DHU arm stem was replaced with 12 unpaired nucleotides and formed a simple loop. In the control region, the putative termination-associated sequences (TAS) with hairpin-loop structure and the conserved sequence blocks (CSB) I-Ⅲ were identified and considered to be associated with the replication and transcription of mtDNA. No repeat motifs were found in the mitogenome of C. griseum. On the basis of the mitogenomic data available in the Class Chondrichthyes from both Genbank and this study, and the phylogenetic analyses using maximum likelihood (ML) and Bayesian inference (BI) methods, the results strongly support the basal division between batoids and sharks and the monophyly of the Superorder Galeomorphii, and confirm the phylogenetic position of C. griseum in the Subclass Elasmobranchii. This study suggests that the mitogenomic data are more robust for reconstructing phylogenetic relationships than individual genes in the Elasmobranchii. |
| 中文摘要: |
| The complete mitochondrial genome of the grey bamboo shark, Chiloscyllium griseum (Orectolobiformes:Hemiscylliidae) was obtained, with a total of 16 755 base pairs (bp). The mitogenome encoded 13 proteincoding genes, two ribosomal RNAs, 22 transfer RNAs and a noncoding control region, a gene arrangement identical to the most common type found in vertebrates. All tRNA sequences were folded into typical cloverleaf secondary structure with the exception of tRNA-Ser2, in which the DHU arm stem was replaced with 12 unpaired nucleotides and formed a simple loop. In the control region, the putative termination-associated sequences (TAS) with hairpin-loop structure and the conserved sequence blocks (CSB) I-Ⅲ were identified and considered to be associated with the replication and transcription of mtDNA. No repeat motifs were found in the mitogenome of C. griseum. On the basis of the mitogenomic data available in the Class Chondrichthyes from both Genbank and this study, and the phylogenetic analyses using maximum likelihood (ML) and Bayesian inference (BI) methods, the results strongly support the basal division between batoids and sharks and the monophyly of the Superorder Galeomorphii, and confirm the phylogenetic position of C. griseum in the Subclass Elasmobranchii. This study suggests that the mitogenomic data are more robust for reconstructing phylogenetic relationships than individual genes in the Elasmobranchii. |
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