| LIU Chenlin,HUANG Xiaohang,LIU Jianguo. 2013. Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers. Acta Oceanologica Sinica, 32(2):59－65 |
| Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers |
| Assessment of introduced Kappaphycus (Solieriaceae, Rhodophyta) species relationships in China with molecular markers |
| Received:June 23, 2011 Revised:February 20, 2012 |
| DOI：10.1007/s13131-013-0277-5 |
| Key words:18S rDNA cox2-cox3 spacer region molecular marker Kappaphycus rbcL |
| 中文关键词: 18S rDNA cox2-cox3 spacer region molecular marker Kappaphycus rbcL |
| 基金项目:Basic Scientific Fund for National Public Research Institutes of China under contract No. 2008G19. |
| Author Name | Affiliation | E-mail | | LIU Chenlin | Marine Ecological Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | ch.lliu@163.com | | HUANG Xiaohang | Marine Ecological Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | LIU Jianguo | Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China | |
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| Abstract: |
| Due to morphological plasticity and paucity of diagnostic morphological characters, the taxonomy of Kappaphycus gets more and more confused with the expanding of commercial cultivation. In this study, the phylogenetic relationship of 13 strains of introduced Kappaphycus species in China was defined using DNA molecular markers, such as 18S rDNA, rbcL and cox2-cox3 spacer region. The resolutions obtained by three different molecular markers were compared:both cox2-cox3 spacer region and rbcL sequences are eligible in inter-species identification of Kappaphycus, whereas cox2-cox3 spacer region is more variable than rbcL sequence. There is several basepairs' discrepancy among 18S rDNA sequences, while it is 100% identical among both cox2-cox3 spacer region and rbcL sequences of the ten strains of K. alvarezii. We suppose that 18S rDNA sequence can provide more information in biogeography study of Kappaphycus than other two DNA sequences. |
| 中文摘要: |
| Due to morphological plasticity and paucity of diagnostic morphological characters, the taxonomy of Kappaphycus gets more and more confused with the expanding of commercial cultivation. In this study, the phylogenetic relationship of 13 strains of introduced Kappaphycus species in China was defined using DNA molecular markers, such as 18S rDNA, rbcL and cox2-cox3 spacer region. The resolutions obtained by three different molecular markers were compared:both cox2-cox3 spacer region and rbcL sequences are eligible in inter-species identification of Kappaphycus, whereas cox2-cox3 spacer region is more variable than rbcL sequence. There is several basepairs' discrepancy among 18S rDNA sequences, while it is 100% identical among both cox2-cox3 spacer region and rbcL sequences of the ten strains of K. alvarezii. We suppose that 18S rDNA sequence can provide more information in biogeography study of Kappaphycus than other two DNA sequences. |
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