| SUN Yumiao,LI Fuhua,CHI Yanhong,XIANG Jianhai. 2013. Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria. Acta Oceanologica Sinica, 32(2):52－58 |
| Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria |
| Enhanced resistance of marine shrimp Exopalamon carincauda Holthuis to WSSV by injecting live VP28-recombinant bacteria |
| Received:June 29, 2011 Revised:April 09, 2012 |
| DOI：10.1007/s13131-013-0261-0 |
| Key words:WSSV VP28 Exopalamon carincauda live bacterial deliver |
| 中文关键词: WSSV VP28 Exopalamon carincauda live bacterial deliver |
| 基金项目:The National Basic Research Program of China (973 Program) under contract No. 2012CB114403; General Program of National Natural Science Foundation of China to Dr. Fuhua Li under contract No. 31072203; National Hightech Research and Development Program under contract Nos 2012AA10A404 and 2012AA092205; China Agriculture Research System under contract No. CARS-47. |
| Author Name | Affiliation | E-mail | | SUN Yumiao | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate University, Chinese Academy of Sciences, Beijing 100049, China | | | LI Fuhua | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | | | CHI Yanhong | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate University, Chinese Academy of Sciences, Beijing 100049, China | | | XIANG Jianhai | Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | jhxiang@qdio.ac.cn |
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| Abstract: |
| Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. |
| 中文摘要: |
| Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus (WSSV) envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a constitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant protein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrimp. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI (Resistance index) of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 (P <0.05). The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV. |
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