| HE Peiqing,LIU Yan,YUE Wenjuan,HUANG Xiaohang. 2013. Key genes expression of reductive tricarboxylic acid cycle from deep-sea hydrothermal chemolithoautotrophic Caminibacter profundus in response to salinity, pH and O2. Acta Oceanologica Sinica, 32(2):35－41 |
| Key genes expression of reductive tricarboxylic acid cycle from deep-sea hydrothermal chemolithoautotrophic Caminibacter profundus in response to salinity, pH and O2 |
| Key genes expression of reductive tricarboxylic acid cycle from deep-sea hydrothermal chemolithoautotrophic Caminibacter profundus in response to salinity, pH and O2 |
| Received:June 29, 2011 Revised:April 09, 2012 |
| DOI：10.1007/s13131-013-0275-7 |
| Key words:Caminibacter profundus reductive tricarboxylic acid (rTCA) cycle ATP citrate lyase pyruvate:ferredoxin oxidoreductase 2-oxoglutarate:ferredoxin oxidoreductase hydrothermal vent |
| 中文关键词: Caminibacter profundus reductive tricarboxylic acid (rTCA) cycle ATP citrate lyase pyruvate:ferredoxin oxidoreductase 2-oxoglutarate:ferredoxin oxidoreductase hydrothermal vent |
| 基金项目:China Ocean Mineral Resources R&D Association under contract No. DYXM-115-02-2-19; Basic Scientific Fund for National Public Research Institutes of China under contract No. 2011T04. |
| Author Name | Affiliation | E-mail | | HE Peiqing | Key Laboratory of Marine Bioactive Substance, State Oceanic Administration, Qingdao 266061, China
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | LIU Yan | Key Laboratory of Marine Bioactive Substance, State Oceanic Administration, Qingdao 266061, China
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | YUE Wenjuan | Key Laboratory of Marine Bioactive Substance, State Oceanic Administration, Qingdao 266061, China
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | | | HUANG Xiaohang | Key Laboratory of Marine Bioactive Substance, State Oceanic Administration, Qingdao 266061, China
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China | xiaohanghuang@fio.org.cn |
|
| Hits: 761 |
| Download times: 674 |
| Abstract: |
| CO2 fixation pathway of Caminibacter profundus, a chemolithoautotrophic ε-Proteobacteria from deep-sea hydrothermal vent, was determined and characterized by genetic and enzymatic analyses. Gene expression of key enzymes for CO2 fixation in response to salinity, pH and O2 in Medium 829 were also investigated. The results demonstrate that C. profundus contained aclB, porA and oorA, the genes encoding key enzymes of reductive tricarboxylic acid (rTCA) cycle. However, genes fragments of cbbL and cbbM encoding key enzyme of Calvin cycle were not recovered. Key enzymatic activities of ATP citrate lyase (ACL), pyruvate:ferredoxin oxidoreductase (POR) and 2-oxoglutarate:ferredoxin oxidoreductase (OOR) were also present in C. profundus. The combination of genetic and enzymatic analyses confirm that C. profundus adopted rTCA cycle for carbon assimilation. The results of aclB and oorA relative expressions of C. profundus demonstrate that the ranges of environmental factors for high genes expression were sea salt 3.0%-5.0% (optimum 3.0%), pH 5.0-6.5(optimum pH 6.5), anaerobic to microaerobic conditions (optimum 1.0% O2). Gene expression patterns under different conditions show similar patterns with bacterial growth, revealing that key rTCA cycle genes provided molecular basis for bacterial growth and propagation. Our results suggest that C. profundus could regulate key genes of rTCA cycle for carbon assimilation and energy metabolism in response to environmental fluctuations in hydrothermal vent. |
| 中文摘要: |
| CO2 fixation pathway of Caminibacter profundus, a chemolithoautotrophic ε-Proteobacteria from deep-sea hydrothermal vent, was determined and characterized by genetic and enzymatic analyses. Gene expression of key enzymes for CO2 fixation in response to salinity, pH and O2 in Medium 829 were also investigated. The results demonstrate that C. profundus contained aclB, porA and oorA, the genes encoding key enzymes of reductive tricarboxylic acid (rTCA) cycle. However, genes fragments of cbbL and cbbM encoding key enzyme of Calvin cycle were not recovered. Key enzymatic activities of ATP citrate lyase (ACL), pyruvate:ferredoxin oxidoreductase (POR) and 2-oxoglutarate:ferredoxin oxidoreductase (OOR) were also present in C. profundus. The combination of genetic and enzymatic analyses confirm that C. profundus adopted rTCA cycle for carbon assimilation. The results of aclB and oorA relative expressions of C. profundus demonstrate that the ranges of environmental factors for high genes expression were sea salt 3.0%-5.0% (optimum 3.0%), pH 5.0-6.5(optimum pH 6.5), anaerobic to microaerobic conditions (optimum 1.0% O2). Gene expression patterns under different conditions show similar patterns with bacterial growth, revealing that key rTCA cycle genes provided molecular basis for bacterial growth and propagation. Our results suggest that C. profundus could regulate key genes of rTCA cycle for carbon assimilation and energy metabolism in response to environmental fluctuations in hydrothermal vent. |
|
HTML
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
