| YE Hua,REN Peng,ZHAO Guangtai,YUE Genhua,WANG Zhiyong. 2012. Isolation and characterization of polymorphic microsatellite loci in large yellow croaker, Larimichthys crocea. Acta Oceanologica Sinica, (4):149－153 |
| Isolation and characterization of polymorphic microsatellite loci in large yellow croaker, Larimichthys crocea |
| Isolation and characterization of polymorphic microsatellite loci in large yellow croaker, Larimichthys crocea |
| Received:January 24, 2011 Revised:March 31, 2012 |
| DOI：10.1007/s13131-012-0228-6 |
| Key words:Larimichthys crocea microsatellites microsatellite-enriched library genetic diversity |
| 中文关键词: Larimichthys crocea microsatellites microsatellite-enriched library genetic diversity |
| 基金项目:The Special Fund for Agro-Scientific Research in the Public Interest under contract No. 200903046-5; the National High Technology Research and Development Program of China (863 program) under contract No. 2006AA10A405; the National Natural Science Foundation of China under contract No. 30771663; the Foundation for Innovation Research Team of Jimei University, China under contract No. 2006A001. |
| Author Name | Affiliation | E-mail | | YE Hua | Fisheries Breeding and Healthy Cultivation Research Centre, Southwest University, Chongqing 402460, China
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Jimei University, Xiamen 361021, China | | | REN Peng | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Jimei University, Xiamen 361021, China | | | ZHAO Guangtai | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Jimei University, Xiamen 361021, China | | | YUE Genhua | Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 117604 Singapore | | | WANG Zhiyong | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Jimei University, Xiamen 361021, China | zywang@jmu.edu.cn |
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| Abstract: |
| An (AC)n-microsatellite-enriched library for Larimichthys crocea was constructed in this study. Primers for fifty simple sequence repeat (SSR) loci were synthesized and genotyped on 30 L. crocea individuals from Guanjingyang wild population (WP) in Fujian Province and 38 individuals from Ningbo cultured population (CP) in Zhejiang Province. Only 21 loci were successfully amplified and polymorphic in two populations. In WP, the observed heterozygosity (HO) ranged from 0.233 to 0.900 and the expected heterozygosity (HE) ranged from 0.326 to 0.893, with an average of 7.8 alleles/locus, the polymorphism information content (PIC) ranged from 0.283 to 0.866 (mean 0.731). In CP, the HO ranged from 0.189 to 0.892 and the HE ranged from 0.333 to 0.800, with an average of 4.4 alleles/locus. The probability test showed significant departures from HWE in 9 and 2 of the 21 loci in WP and in CP, respectively. Deficiency of heterozygotes at four loci showed the presence of null alleles (P <0.01). The PIC of 20 microsatellite loci in WP were greater than 0.50. Overall, these novel markers are potentially useful for the study of population genetics, construction of genetic linkage and quantitative trait loci maps in large yellow croaker. |
| 中文摘要: |
| An (AC)n-microsatellite-enriched library for Larimichthys crocea was constructed in this study. Primers for fifty simple sequence repeat (SSR) loci were synthesized and genotyped on 30 L. crocea individuals from Guanjingyang wild population (WP) in Fujian Province and 38 individuals from Ningbo cultured population (CP) in Zhejiang Province. Only 21 loci were successfully amplified and polymorphic in two populations. In WP, the observed heterozygosity (HO) ranged from 0.233 to 0.900 and the expected heterozygosity (HE) ranged from 0.326 to 0.893, with an average of 7.8 alleles/locus, the polymorphism information content (PIC) ranged from 0.283 to 0.866 (mean 0.731). In CP, the HO ranged from 0.189 to 0.892 and the HE ranged from 0.333 to 0.800, with an average of 4.4 alleles/locus. The probability test showed significant departures from HWE in 9 and 2 of the 21 loci in WP and in CP, respectively. Deficiency of heterozygotes at four loci showed the presence of null alleles (P <0.01). The PIC of 20 microsatellite loci in WP were greater than 0.50. Overall, these novel markers are potentially useful for the study of population genetics, construction of genetic linkage and quantitative trait loci maps in large yellow croaker. |
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