| WENG Zhaohong,XIAO Zhiqun,XIE Yangjie,WANG Zhiyong,GUI Jianfang. 2012. Genetic difference of Chinese horseshoe crab (Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis. Acta Oceanologica Sinica, (3):132－137 |
| Genetic difference of Chinese horseshoe crab (Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis |
| Genetic difference of Chinese horseshoe crab (Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis |
| Received:May 23, 2011 Revised:October 10, 2011 |
| DOI：10.1007/s13131-012-0213-0 |
| Key words:Tachypleus tridentatus genetic difference cytochrome c oxidase subunit I gene mtDNA |
| 中文关键词: Tachypleus tridentatus genetic difference cytochrome c oxidase subunit I gene mtDNA |
| 基金项目:F5 Subject from Science and Technology Department of Fujian Province under contract No. 2008F5038; the Open Foundation from Ocean Fishery Science and Technology in the Most Important Subjects of Zhejiang under contract No. 20100210. |
| Author Name | Affiliation | E-mail | | WENG Zhaohong | Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Fisheries College, Jimei University, Xiamen 361021, China
Graduate School of Chinese Academy of Sciences, Beijing 100039, China | | | XIAO Zhiqun | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Fisheries College, Jimei University, Xiamen 361021, China | | | XIE Yangjie | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Fisheries College, Jimei University, Xiamen 361021, China | | | WANG Zhiyong | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture of the P. R. C., Fisheries College, Jimei University, Xiamen 361021, China | | | GUI Jianfang | Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China | jfgui@ihb.ac.cn |
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| Abstract: |
| Population genetic structure and historical demography of Chinese horseshoe crab (T. tridentatus) along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit I (COI) fragment. The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province, Meizhou and Zhangpu in Fujian Province, Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province. Sequence variation was relatively low with a total of seven transitions observed. In all localities, Haplotype H3 was the dominant type observed among eight haplotypes defined previously, and was at the center of radiation in Median-Joining network. The prolonged star-like network suggests a signature of population expansions. The level of diversity was low in total, with haplotype diversity (Hd) being equal to 0.765 and nucleotide diversity (π) being equal to 0.001 18, respectively. The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations. Both mismatch distribution analysis and Fu's Fs test provided consistent inference of historic population expansion. The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal. |
| 中文摘要: |
| Population genetic structure and historical demography of Chinese horseshoe crab (T. tridentatus) along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit I (COI) fragment. The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities:Ninghai in Zhejiang Province, Meizhou and Zhangpu in Fujian Province, Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province. Sequence variation was relatively low with a total of seven transitions observed. In all localities, Haplotype H3 was the dominant type observed among eight haplotypes defined previously, and was at the center of radiation in Median-Joining network. The prolonged star-like network suggests a signature of population expansions. The level of diversity was low in total, with haplotype diversity (Hd) being equal to 0.765 and nucleotide diversity (π) being equal to 0.001 18, respectively. The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations. Both mismatch distribution analysis and Fu's Fs test provided consistent inference of historic population expansion. The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal. |
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