| KAN Guangfeng,SHI Cuijuan,WANG Xiaofei,XIE Qiuju,WANG Min,WANG Xinlei,MIAO Jinlai. 2012. Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica. Acta Oceanologica Sinica, (1):116－124 |
| Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica |
| Acclimatory responses to high-salt stress in Chlamydomonas (Chlorophyta, Chlorophyceae) from Antarctica |
| Received:December 04, 2010 Revised:October 11, 2011 |
| DOI：10.1007/s13131-012-0183-2 |
| Key words:Antarctic ice microalga SOD enzymes ultra structure membrane fatty acids difference protein hypersaline acclimation |
| 中文关键词: Antarctic ice microalga SOD enzymes ultra structure membrane fatty acids difference protein hypersaline acclimation |
| 基金项目:The National Natural Science Foundation of China under contract No. 31100090; Shandong Provincial Natural Science Foundation of China under contract No. ZR2010DQ010; the Fundamental Research Funds for the Central Universities under contract No. HIT.IBRSEM.2009148. |
| Author Name | Affiliation | E-mail | | KAN Guangfeng | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | kanguangfeng@163.com | | SHI Cuijuan | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | | | WANG Xiaofei | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | | | XIE Qiuju | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | | | WANG Min | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | | | WANG Xinlei | School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai 264209, China | | | MIAO Jinlai | Key Laboratory of Marine Bio-active Substances, State Oceanic Administration, Qingdao 266061, China | |
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| Abstract: |
| Antarctic ice microalga can survive and thrive in channels or pores containing high salinity in Antarctic ice layer. In this study, it was found that cell membrane permeability of green microalga Chlamydomonas sp. L4 from Antarctic sea ice was high in cells treated with hypersalinity due to the induction of active oxygen and radicals. However, increased super oxide dismutase (SOD) scavenged harmful free radicals effectively to keep cell membrane integrity. Also, the analysis of membrane fatty acids demonstrated the content of saturated fatty acids and monounsaturated fatty acids increased and polyunsaturated fatty acids decreased under the high-salt treatment for 14 d, which effectively reduced the membrane fluidity and minimized the injury to cell membrane. The morphological changes showed that hypersalinity induced the increase of cell volume and the consumption of starch granules. However, because of the increase in detoxification of vacuoles, electron-dense deposits and SOD activity under high-salt stress, the complete noninterference thylakoids, mitochondria and cell nucleus maintained cellular fundamental metabolism. Global-expression profiling of proteins showed eight protein spots disappeared, 18 protein spots decreased and 18 protein spots were enhanced after the high-salt shock obviously (P <0.05). One new peptide (pI 6.90; MW 51 kDa) was primarily confirmed as the processor of light reaction center protein CP43 in photosystem Ⅱ, which increased photosynthesis ability of Chlamydomonas sp. L4 treated with high salinity. |
| 中文摘要: |
| Antarctic ice microalga can survive and thrive in channels or pores containing high salinity in Antarctic ice layer. In this study, it was found that cell membrane permeability of green microalga Chlamydomonas sp. L4 from Antarctic sea ice was high in cells treated with hypersalinity due to the induction of active oxygen and radicals. However, increased super oxide dismutase (SOD) scavenged harmful free radicals effectively to keep cell membrane integrity. Also, the analysis of membrane fatty acids demonstrated the content of saturated fatty acids and monounsaturated fatty acids increased and polyunsaturated fatty acids decreased under the high-salt treatment for 14 d, which effectively reduced the membrane fluidity and minimized the injury to cell membrane. The morphological changes showed that hypersalinity induced the increase of cell volume and the consumption of starch granules. However, because of the increase in detoxification of vacuoles, electron-dense deposits and SOD activity under high-salt stress, the complete noninterference thylakoids, mitochondria and cell nucleus maintained cellular fundamental metabolism. Global-expression profiling of proteins showed eight protein spots disappeared, 18 protein spots decreased and 18 protein spots were enhanced after the high-salt shock obviously (P <0.05). One new peptide (pI 6.90; MW 51 kDa) was primarily confirmed as the processor of light reaction center protein CP43 in photosystem Ⅱ, which increased photosynthesis ability of Chlamydomonas sp. L4 treated with high salinity. |
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