| ZHU Zewen,XU Ting,HE Zhongyang,WU Xinzhong,WU Liuji,MENG Qingguo,HUANG Jiaqing. 2012. Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China. Acta Oceanologica Sinica, (1):106－115 |
| Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China |
| Rickettsia-like organism infection associated with mass mortalities of blood clam, Tegillarca granosa, in the Yueqing Bay in China |
| Received:October 30, 2010 Revised:April 23, 2011 |
| DOI：10.1007/s13131-012-0182-3 |
| Key words:blood clam Tegillarca granosa rickettsia-like organism (RLO) inclusions mass mortalities |
| 中文关键词: blood clam Tegillarca granosa rickettsia-like organism (RLO) inclusions mass mortalities |
| 基金项目:Scientific Projects of Zhejiang Province, China under contract Nos 2004C23041 and 20080123. |
| Author Name | Affiliation | E-mail | | ZHU Zewen | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | | | XU Ting | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | | | HE Zhongyang | Fisheries Technical Extension Center of Zhejiang Province, Hangzhou 310012, China | | | WU Xinzhong | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | wuxz@zju.edu.cn | | WU Liuji | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | | | MENG Qingguo | Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China | | | HUANG Jiaqing | Fisheries Technical Extension Center of Zhejiang Province, Hangzhou 310012, China | |
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| Abstract: |
| A series of mass mortalities of the cultured blood clam, Tegillarca granosa, occurred in the Yueqing Bay of China from 2005 to 2009. An obligate intracellular prokaryote, designated as rickettsia-like organism (RLO), was frequently found in the moribund or dead blood clam sample during ultrastructural examination. These organisms were usually round, ellipsoid or occasionally dumbbellshaped, ranged from approximately 0.28 to 0.71 μm in size and had a trilaminar cell wall. Two reproductive modes of organisms, transverse binary fission and budding, were observed. The organisms were able to form eosinophilic inclusions. Most inclusions were found within epithelial and connective tissues of the mantle, gills and digestive tube. The biological and morphological characteristics indicate that these organisms may belong to the family Rickettsiaceae. RLOs exhibited significant pathogenicity. Cytopathological examinations revealed extensive necrosis and destruction in the infected cell. The degree of tissue destruction was positively related to the number of RLO inclusions in the tissues, and the cytopathological effects were positively related to the number of intracellular RLO. RLOs and their inclusions were discovered throughout different disease areas and in different time periods. The infection intensity of the RLOs was positively correlated with the mortality rate of clams. Therefore, RLO infection might be associated with mass mortalities of cultured blood clams in the Yueqing Bay. |
| 中文摘要: |
| A series of mass mortalities of the cultured blood clam, Tegillarca granosa, occurred in the Yueqing Bay of China from 2005 to 2009. An obligate intracellular prokaryote, designated as rickettsia-like organism (RLO), was frequently found in the moribund or dead blood clam sample during ultrastructural examination. These organisms were usually round, ellipsoid or occasionally dumbbellshaped, ranged from approximately 0.28 to 0.71 μm in size and had a trilaminar cell wall. Two reproductive modes of organisms, transverse binary fission and budding, were observed. The organisms were able to form eosinophilic inclusions. Most inclusions were found within epithelial and connective tissues of the mantle, gills and digestive tube. The biological and morphological characteristics indicate that these organisms may belong to the family Rickettsiaceae. RLOs exhibited significant pathogenicity. Cytopathological examinations revealed extensive necrosis and destruction in the infected cell. The degree of tissue destruction was positively related to the number of RLO inclusions in the tissues, and the cytopathological effects were positively related to the number of intracellular RLO. RLOs and their inclusions were discovered throughout different disease areas and in different time periods. The infection intensity of the RLOs was positively correlated with the mortality rate of clams. Therefore, RLO infection might be associated with mass mortalities of cultured blood clams in the Yueqing Bay. |
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