| ZHAO Jing,YANG Ning,CHEN Xinglin,JIANG Qingru,ZENG Runying. 2011. Phylogenetic diversity of Type I polyketide synthase genes from sediments of Ardley Island in Antarctica. Acta Oceanologica Sinica, (6):104－111 |
| Phylogenetic diversity of Type I polyketide synthase genes from sediments of Ardley Island in Antarctica |
| Phylogenetic diversity of Type I polyketide synthase genes from sediments of Ardley Island in Antarctica |
| Received:November 17, 2010 Revised:March 25, 2011 |
| DOI：10.1007/s13131-011-0167-7 |
| Key words:polyketide synthase diversity Antarctica |
| 中文关键词: polyketide synthase diversity Antarctica |
| 基金项目:The National Natural Science Foundation of China under contract No. 40406029; China Ocean Mineral Resources Research & Development Association Funds under contract No. DYXM-115-02-2-04. |
| Author Name | Affiliation | E-mail | | ZHAO Jing | Department of Marine Technology and Ocean Engineering, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | | | YANG Ning | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | CHEN Xinglin | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | | | JIANG Qingru | Department of Marine Technology and Ocean Engineering, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China | | | ZENG Runying | Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China | runyingzeng@yahoo.com.cn |
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| Abstract: |
| The diversity of modular polyketide synthase (PKS) genes in sediments of Ardley Island in Antarctica, was studied by restriction fragment length polymorphism (RFLP) analysis. Phylogenetic analysis of 14 amino acid (AA) sequences indicates that the identified ketosynthase (KS) domains were clustered with those from diverse bacterial groups, including Cyanobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, and some unidentified microorganisms from marine sponge, bryozoan and other environmental samples. The obtained KS domains showed 43%-81% similarity at the AA level to reference sequences in GenBank. Six identified KS domains showed diverse sequences of the motif (VQTACSTS) that was used to identify the hybrid PKS/nonribosomal peptide synthetase (NRPS) enzyme complex, and formed a new branch. These results reveal a high diversity and novelty of PKS genes in antarctic sediments. |
| 中文摘要: |
| The diversity of modular polyketide synthase (PKS) genes in sediments of Ardley Island in Antarctica, was studied by restriction fragment length polymorphism (RFLP) analysis. Phylogenetic analysis of 14 amino acid (AA) sequences indicates that the identified ketosynthase (KS) domains were clustered with those from diverse bacterial groups, including Cyanobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, and some unidentified microorganisms from marine sponge, bryozoan and other environmental samples. The obtained KS domains showed 43%-81% similarity at the AA level to reference sequences in GenBank. Six identified KS domains showed diverse sequences of the motif (VQTACSTS) that was used to identify the hybrid PKS/nonribosomal peptide synthetase (NRPS) enzyme complex, and formed a new branch. These results reveal a high diversity and novelty of PKS genes in antarctic sediments. |
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