| LIU Jingwen,Gunnar Bratbak,ZHENG Tianling,Runar Thyrhaug. 2011. Effects of virus infection on expression of cell cycle regulatory proteins in the unicellular marine algae Emiliania huxleyi. Acta Oceanologica Sinica, (4):89－95 |
| Effects of virus infection on expression of cell cycle regulatory proteins in the unicellular marine algae Emiliania huxleyi |
| Effects of virus infection on expression of cell cycle regulatory proteins in the unicellular marine algae Emiliania huxleyi |
| Received:September 12, 2010 Revised:December 01, 2010 |
| DOI：10.1007/s13131-011-0138-z |
| Key words:marine algal virus cell cycle regulators expression Emiliania huxleyi |
| 中文关键词: marine algal virus cell cycle regulators expression Emiliania huxleyi |
| 基金项目:The National High Technology Research and Development Program of China under contract No. 2008AA09Z408; the National Natural Science Foundation of China under contract Nos 40930847, 30940002 and 40876061; Fujian Province Natural Science Foundation, China under contract No. 2010J01261; the Foundation for Innovative Research Team of Jimei University, China under contract No. 2010A007. |
| Author Name | Affiliation | E-mail | | LIU Jingwen | Bioengineering College of Jimei University, Xiamen 361021, China | ljwsbch@163.com | | Gunnar Bratbak | Department of Microbiology, University of Bergen, Bergen N-5020, Norway | | | ZHENG Tianling | Key Laboratory of MOE for Coast and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen 361005, China
State Key Laboratory of Marine Environmental Sciences of Xiamen University, Xiamen 361005, China | | | Runar Thyrhaug | Department of Microbiology, University of Bergen, Bergen N-5020, Norway | |
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| Abstract: |
| The authors have investigated the biochemical events by which marine algal virus infection induces cell cycle arrest. The key G2/M-phase regulatory proteins are analyzed by immunobloting in unicellular Emiliania huxleyi, suggesting that virus induced cell cycle arrest is related with virus's effect on cyclins and cyclin dependent kinases. E. huxleyi virus (EhV) represses Cdc2/cyclinB complex activity by inhibiting the activity of Cdc2 kinase in a phosphorylation-related manner, blocking host cells G2/M checkpoint. Dephosphorylated/inactive Cdc25C combined with up-regulation of Wee1 expression at early infect period appears to be important mechanisms by which EhV represses Cdc2/cyclinB complex activity that is required for entry into M phase. This study has allowed us to confirm that algal virus infection leads to selective activation or inhibition of certain cell-cycle factors, which may play a significant role in establishing a more efficient environment for viral gene expression and DNA replication. |
| 中文摘要: |
| The authors have investigated the biochemical events by which marine algal virus infection induces cell cycle arrest. The key G2/M-phase regulatory proteins are analyzed by immunobloting in unicellular Emiliania huxleyi, suggesting that virus induced cell cycle arrest is related with virus's effect on cyclins and cyclin dependent kinases. E. huxleyi virus (EhV) represses Cdc2/cyclinB complex activity by inhibiting the activity of Cdc2 kinase in a phosphorylation-related manner, blocking host cells G2/M checkpoint. Dephosphorylated/inactive Cdc25C combined with up-regulation of Wee1 expression at early infect period appears to be important mechanisms by which EhV represses Cdc2/cyclinB complex activity that is required for entry into M phase. This study has allowed us to confirm that algal virus infection leads to selective activation or inhibition of certain cell-cycle factors, which may play a significant role in establishing a more efficient environment for viral gene expression and DNA replication. |
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