| WANG Guoliang,TAN Xiaolong,SHEN Junling,LI Jian,ZHANG Lei,SUN Jianwei,WANG Bin,WENG Manli,LIU Tao. 2011. Development of EST-SSR primers and their practicability test for Laminaria. Acta Oceanologica Sinica, (3):112－117 |
| Development of EST-SSR primers and their practicability test for Laminaria |
| Development of EST-SSR primers and their practicability test for Laminaria |
| Received:May 10, 2010 Revised:November 19, 2010 |
| DOI：10.1007/s13131-011-0125-4 |
| Key words:Laminaria EST-SSRs germplasm identification polymorphism analysis |
| 中文关键词: Laminaria EST-SSRs germplasm identification polymorphism analysis |
| 基金项目:The National Natural Science Foundation of China under contract No. 40776072 and 40606035; the National High Technology Research and Development Program (863 Program) of China under contract Nos 2006AA10A412 and 2010AA10A401; the Key Projects in the National Science & Technology Pillar Program under contract No. 2006BAD01A13. |
| Author Name | Affiliation | E-mail | | WANG Guoliang | College of Marine Life Science, Ocean University of China, Qingdao 266003, China
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China | | | TAN Xiaolong | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | SHEN Junling | College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China | | | LI Jian | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | ZHANG Lei | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | | | SUN Jianwei | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China | | | WANG Bin | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China | | | WENG Manli | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China | | | LIU Tao | College of Marine Life Science, Ocean University of China, Qingdao 266003, China | liutao@ouc.edu.cn |
|
| Hits: 736 |
| Download times: 671 |
| Abstract: |
| Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data. |
| 中文摘要: |
| Laminaria (Saccharina) is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data. |
|
HTML
View Full Text
View/Add Comment Download reader |
| Close |
|
|
|
