| LI Jialin,BAI Jie,GAO Huiwang,LIU Guangxing. 2011. Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer. Acta Oceanologica Sinica, (3):92－99 |
| Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer |
| Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer |
| Received:May 31, 2010 Revised:January 20, 2011 |
| DOI：10.1007/s13131-011-0123-6 |
| Key words:ammonia-oxidizing Betaproteobacteria (βAOB) diversity abundance nitrification surface sediment Changjiang River Estuary (CRE) |
| 中文关键词: ammonia-oxidizing Betaproteobacteria (βAOB) diversity abundance nitrification surface sediment Changjiang River Estuary (CRE) |
| 基金项目:The National Fundamental Project of China under grant No. 2006CB400602. |
| Author Name | Affiliation | E-mail | | LI Jialin | Key Laboratory of Marine Environmental Science and Ecology of Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China | | | BAI Jie | Key Laboratory of Marine Environmental Science and Ecology of Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | baijie@ouc.edu.cn | | GAO Huiwang | Key Laboratory of Marine Environmental Science and Ecology of Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | | | LIU Guangxing | Key Laboratory of Marine Environmental Science and Ecology of Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China | |
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| Abstract: |
| The spatial distribution of ammonia-oxidizing Betaproteobacteria (βAOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of βAOB ranged from 1.87×105 to 3.53×105 cells/g of sediment. βAOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the βAOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of βAOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the βAOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between βAOB abundance and nitrification estimates, the abundance and diversity of βAOB community could be expected as an indirect index of nitrification activity at the study sea area in summer. |
| 中文摘要: |
| The spatial distribution of ammonia-oxidizing Betaproteobacteria (βAOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary. Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006. The abundance of βAOB ranged from 1.87×105 to 3.53×105 cells/g of sediment. βAOB abundance did not present a negative correlation with salinity, whereas salinity was implicated as the primary factor affecting nitrification rates. The DGGE profiles of PCR-amplified amoA gene fragments revealed that the βAOB community structure of sample S2 separated from other samples at the level of 40% similarity. The variations in composition of βAOB were significantly correlated with the salinity, temperature, absorption ability of sediments and TOC. The statistical analysis indicates that the βAOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity. Considering the good correlation between βAOB abundance and nitrification estimates, the abundance and diversity of βAOB community could be expected as an indirect index of nitrification activity at the study sea area in summer. |
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