| WU Changwen,CHI Changfeng,HE Guangyuan,LÜ Zhenming,XU Meiying. 2010. Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns. Acta Oceanologica Sinica, (6):121－124 |
| Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns |
| Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns |
| Received:September 02, 2009 Revised:April 20, 2010 |
| DOI：10.1007/s13131-010-0083-2 |
| Key words:Sepiella maindroni microsatellite DNA cuttlefish genetic variation |
| 中文关键词: Sepiella maindroni microsatellite DNA cuttlefish genetic variation |
| 基金项目:The National Key Technology R & D Program of China under contract No. 2007BAD43B05; the Key Science & Technology Specific Projects of Zhejiang Province under contract No. 2007C12062; the National Natural Science Foundation of China under contract No. 31001109 |
| Author Name | Affiliation | E-mail | | WU Changwen | Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China
Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China | wcwccf@hotmail.com | | CHI Changfeng | Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China | | | HE Guangyuan | Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China | | | LÜ Zhenming | Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China | | | XU Meiying | Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China
Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China | |
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| Abstract: |
| Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni. |
| 中文摘要: |
| Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni. |
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