| LIU Feng,PANG Shaojun. 2010. Nonculturability of the pathogenic Vibrio parahaemolyticus in live culture of Grateloupia turuturu is associated with bacterial attachment to the algal thalli. Acta Oceanologica Sinica, (6):92－103 |
| Nonculturability of the pathogenic Vibrio parahaemolyticus in live culture of Grateloupia turuturu is associated with bacterial attachment to the algal thalli |
| Nonculturability of the pathogenic Vibrio parahaemolyticus in live culture of Grateloupia turuturu is associated with bacterial attachment to the algal thalli |
| Received:September 22, 2009 Revised:April 22, 2010 |
| DOI：10.1007/s13131-010-0080-5 |
| Key words:Grateloupia turuturu attachment Vibrio parahaemolyticus nonculturability effective quantum yield |
| 中文关键词: Grateloupia turuturu attachment Vibrio parahaemolyticus nonculturability effective quantum yield |
| 基金项目:The National Natural Science Foundation of China under contract No. 30671596. |
| Author Name | Affiliation | E-mail | | LIU Feng | Marine Biological Culture Collection Centre, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Graduate University of Chinese Academy of Sciences, Beijing 100049, China | | | PANG Shaojun | Marine Biological Culture Collection Centre, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China | sjpang@ms.qdio.ac.cn |
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| Abstract: |
| The invasive red alga Grateloupia turuturu Yamada could turn Vibrio parahaemolyticus into nonculturable state in live algal culture. In order to elucidate the mechanism of such an effect, a series of culture experiments were performed in this investigation based on three hypothesized causes, namely bacterial attachment, production of reactive oxygen species (ROS) and the discharge of water soluble secondary metabolic compounds. The results reveal that attachment to the thallus surface of G. turuturu was the major reason for the decrease of V. parahaemolyticus in seawater. Further investigations show that V. parahaemolyticus attachment to the surface of algal thallus in live cultures of seaweeds was a common phenomenon. However, the disappearance of the culturability of V. parahaemolyticus occurred only on the thallus of G. turuturu over 72 h among all six algal species tested. Electron microscopic scanning shows that most of V. parahaemolyticus attached to G. turuturu changed from the initial normal bacilli to coccoid-shape after 72 h. The enclosure experiments by enclosing the algal thallus in tubes demonstrate that the nonculturability of V. parahaemolyticus in the water of live culture of G. turuturu occurred after the physical contact of the V. parahaemolyticus to the alga. The capacity of G. turuturu in affecting the culturability of V. parahaemolyticus was not influenced after inhibition of photosynthesis by treatment of 3'-(3,4-dichlorophenyl)-1',1'-dimethyl urea (DCMU) at non-lethal levels. Production of reactive oxygen species after addition of live culture of bacteria was excluded by on-line analyzing the oxidation of dichlorohydrofluorescein (DCFH) to dichlorofluorescein (DCF) in the presence of peroxidase on a VersaFluor fluorometer. |
| 中文摘要: |
| The invasive red alga Grateloupia turuturu Yamada could turn Vibrio parahaemolyticus into nonculturable state in live algal culture. In order to elucidate the mechanism of such an effect, a series of culture experiments were performed in this investigation based on three hypothesized causes, namely bacterial attachment, production of reactive oxygen species (ROS) and the discharge of water soluble secondary metabolic compounds. The results reveal that attachment to the thallus surface of G. turuturu was the major reason for the decrease of V. parahaemolyticus in seawater. Further investigations show that V. parahaemolyticus attachment to the surface of algal thallus in live cultures of seaweeds was a common phenomenon. However, the disappearance of the culturability of V. parahaemolyticus occurred only on the thallus of G. turuturu over 72 h among all six algal species tested. Electron microscopic scanning shows that most of V. parahaemolyticus attached to G. turuturu changed from the initial normal bacilli to coccoid-shape after 72 h. The enclosure experiments by enclosing the algal thallus in tubes demonstrate that the nonculturability of V. parahaemolyticus in the water of live culture of G. turuturu occurred after the physical contact of the V. parahaemolyticus to the alga. The capacity of G. turuturu in affecting the culturability of V. parahaemolyticus was not influenced after inhibition of photosynthesis by treatment of 3'-(3,4-dichlorophenyl)-1',1'-dimethyl urea (DCMU) at non-lethal levels. Production of reactive oxygen species after addition of live culture of bacteria was excluded by on-line analyzing the oxidation of dichlorohydrofluorescein (DCFH) to dichlorofluorescein (DCF) in the presence of peroxidase on a VersaFluor fluorometer. |
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