| YE Hua,WANG Xiaoqing,GAO Tianxiang,WANG Zhiyong. 2010. EST-derived microsatellites in Pseudosciaena crocea and their applicability to related species. Acta Oceanologica Sinica, (6):83－91 |
| EST-derived microsatellites in Pseudosciaena crocea and their applicability to related species |
| EST-derived microsatellites in Pseudosciaena crocea and their applicability to related species |
| Received:April 08, 2010 Revised:October 08, 2010 |
| DOI：10.1007/s13131-010-0079-y |
| Key words:Pseudosciaena crocea EST-SSRs genetic diversity cross-species amplification |
| 中文关键词: Pseudosciaena crocea EST-SSRs genetic diversity cross-species amplification |
| 基金项目:The National High Technology Research and Development Program of China (863 program) under contract Nos 2006AA10A405 and 2006AA09Z418; the National Natural Science Foundation of China under contract Nos 30771663 and 30471329; the Foundation for Innovation Research Team of Jimei University, China under contract No. 2006A001. |
| Author Name | Affiliation | E-mail | | YE Hua | College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China | | | WANG Xiaoqing | College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China | | | GAO Tianxiang | Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China | | | WANG Zhiyong | Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China | zywang@jmu.edu.cn |
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| Abstract: |
| Simple sequence repeat (SSR) markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags (ESTs) from the NCBI database. Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China. Sixteen of the loci were polymorphic, which were amplified with 3 to 11 alleles per locus and the mean of 6.13. The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 (mean 0.544) and from 0.118 to 0.892 (mean 0.644), respectively. Polymorphic information content (PIC) ranged from 0.115 to 0.866 (mean 0.593). The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P. polyactis, C. niveatus, C. lucidus, A. argentatus and J. belengeri revealed that 14, 12, 11, 7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus, respectively, which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species. Overall, this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species. |
| 中文摘要: |
| Simple sequence repeat (SSR) markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags (ESTs) from the NCBI database. Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China. Sixteen of the loci were polymorphic, which were amplified with 3 to 11 alleles per locus and the mean of 6.13. The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 (mean 0.544) and from 0.118 to 0.892 (mean 0.644), respectively. Polymorphic information content (PIC) ranged from 0.115 to 0.866 (mean 0.593). The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P. polyactis, C. niveatus, C. lucidus, A. argentatus and J. belengeri revealed that 14, 12, 11, 7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus, respectively, which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species. Overall, this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species. |
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