| SUN Wei,DAI Shikun,WANG Guanghua,XIE Lianwu,JIANG Shumei,LI Xiang. 2010. Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea. Acta Oceanologica Sinica, 29(5):65－73 |
| Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea |
| Phylogenetic diversity of bacteria associated with the marine sponge Agelas robusta from South China Sea |
| Received:June 30, 2009 Revised:May 11, 2010 |
| DOI：10.1007/s13131-010-0064-5 |
| Key words:South China Sea Agelas robusta phylogenetic diversity sponge-specific bacteria |
| 中文关键词: South China Sea Agelas robusta phylogenetic diversity sponge-specific bacteria |
| 基金项目:This research was partially funded by the State Principal and Basic Research and Development Program of the Ministry of Sciences and Technology of China under contract No. 2010CB833801; the Provincial Collaborative Foundation Project of Guangdong under contract Nos 9351007002000001 and 2008A030203004. |
| Author Name | Affiliation | E-mail | | SUN Wei | Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China | | | DAI Shikun | Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China | | | WANG Guanghua | Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China | | | XIE Lianwu | Central South University of Forestry and Technology, Changsha 410004, China | | | JIANG Shumei | Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China | | | LI Xiang | Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB-CAS), Guangdong Key Laboratory of Marine Materia Medica(LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China | lixiang@scsio.ac.cn |
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| Abstract: |
| It is well known that marine sponges harbor large numbers of bacteria, some of which have been proved to be sponge-specific. The diversity of bacteria in marine sponges distributed along the coast of South China Sea has been previously studied but that of bacteria in sponges inhabiting the open sea has been rarely investigated. In this report, the diversity of bacteria associated with the marine sponge Agelas robusta from a remote coral reef in the South China Sea was documented employing 16S rDNA library construction, amplified ribosomal DNA restriction analysis (ARDRA) and phylogenetic analysis. A total of 32 phylotypes were finally categorized in nine phyla including Cyanobacteria, Proteobacteria, Chloroflexi, Firmicutes, Actinobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Gemmatimonadetes. The dominant phylum was Proteobacteria whereas the dominant genus was Synechococcus in Cyanobacteria. Some spongespecific bacteria were also found in the bacteria population, but the proportion (5 OTUs/32 OTUs) was much lower than other sponges. This study reveals the phylogenetic diversity of bacteria in A. robusta and confirms the presence of some sponge-specific bacteria in the South China Sea. Understanding the diversity of sponge-associated bacteria in China assists to exploit the bacteria resources for biotechnology. |
| 中文摘要: |
| It is well known that marine sponges harbor large numbers of bacteria, some of which have been proved to be sponge-specific. The diversity of bacteria in marine sponges distributed along the coast of South China Sea has been previously studied but that of bacteria in sponges inhabiting the open sea has been rarely investigated. In this report, the diversity of bacteria associated with the marine sponge Agelas robusta from a remote coral reef in the South China Sea was documented employing 16S rDNA library construction, amplified ribosomal DNA restriction analysis (ARDRA) and phylogenetic analysis. A total of 32 phylotypes were finally categorized in nine phyla including Cyanobacteria, Proteobacteria, Chloroflexi, Firmicutes, Actinobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Gemmatimonadetes. The dominant phylum was Proteobacteria whereas the dominant genus was Synechococcus in Cyanobacteria. Some spongespecific bacteria were also found in the bacteria population, but the proportion (5 OTUs/32 OTUs) was much lower than other sponges. This study reveals the phylogenetic diversity of bacteria in A. robusta and confirms the presence of some sponge-specific bacteria in the South China Sea. Understanding the diversity of sponge-associated bacteria in China assists to exploit the bacteria resources for biotechnology. |
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